NDLI: Dynamic redistribution of raft domains as an organizing platform for signaling during cell chemotaxis

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Dynamic redistribution of raft domains as an organizing platform for signaling during cell chemotaxis

Content Provider	Scilit
Author	Carlos, Martínez- A. Gómez-Moutón, Concepción Lacalle, Rosa Ana Mira, Emilia Jiménez-Baranda, Sonia Barber, Domingo F. Carrera, Ana C. Mañes, Santos
Copyright Year	2004
Description	Spatially restricted activation of signaling molecules governs critical aspects of cell migration; the mechanism by which this is achieved nonetheless remains unknown. Using time-lapse confocal microscopy, we analyzed dynamic redistribution of lipid rafts in chemoattractant-stimulated leukocytes expressing glycosyl phosphatidylinositol–anchored green fluorescent protein (GFP-GPI). Chemoattractants induced persistent GFP-GPI redistribution to the leading edge raft (L raft) and uropod rafts of Jurkat, HL60, and dimethyl sulfoxide–differentiated HL60 cells in a pertussis toxin–sensitive, actin-dependent manner. A transmembrane, nonraft GFP protein was distributed homogeneously in moving cells. A GFP-CCR5 chimera, which partitions in L rafts, accumulated at the leading edge, and CCR5 redistribution coincided with recruitment and activation of phosphatidylinositol-3 kinase γ in L rafts in polarized, moving cells. Membrane cholesterol depletion impeded raft redistribution and asymmetric recruitment of PI3K to the cell side facing the chemoattractant source. This is the first direct evidence that lipid rafts order spatial signaling in moving mammalian cells, by concentrating the gradient sensing machinery at the leading edge.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172162/pdf https://core.ac.uk/reader/36015483 http://jcb.rupress.org/content/164/5/759.full.pdf
Ending Page	768
Page Count	10
Starting Page	759
DOI	10.1083/jcb.200309101
Journal	Journal of Cell Biology
Issue Number	5
Volume Number	164
Language	English
Publisher	Rockefeller University Press
Publisher Date	2004-02-23
Access Restriction	Open
Subject Keyword	Cell Biology Lipid Rafts Chemotaxis Chemokine Phosphatidyl Inositol-3 Kinase Cell Polarization Cell Polarity Lipid Raft Journal: Journal of Cell Biology (Vol- 164, Issue- 5)
Content Type	Text

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