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Major histocompatibility complex class II-expressing endothelial cells induce allospecific nonresponsiveness in naive T cells.
| Content Provider | Scilit |
|---|---|
| Author | Marelli-Berg, F. M. Hargreaves, R. E. Carmichael, P. Dorling, A. Lombardi, G. Lechler, R. I. |
| Copyright Year | 1996 |
| Description | Journal: The Journal of experimental medicine The role of endothelial cells (EC) in initiating a primary T cell response is of importance in clinical transplantation and autoimmunity since EC are the first allogeneic target encountered by the recipient's immune system and may display tissue-specific autoantigens in the context of an inflammatory response. In this study, we have investigated the antigen-presenting cell function of human umbilical vein-derived EC (HUVEC), depleted of constitutively major histocompatibility complex class II+ cells and induced to express class II molecules by interferon-gamma. The results show that HUVEC do not express B7 but can support proliferation by antigen-specific T cell clones. In contrast, they were unable to initiate a primary alloresponse using three independent HUVEC cultures and MHC class II-mismatched CD4+ T cells from eight donors. The response to HUVEC was reconstituted by trans-costimulation provided by DAP.3 transfectants expressing human B7.1. Coculture of peripheral blood T cells with EC expressing allogeneic DR molecules had markedly different effects on CD45RO+ and RA+ subsets. Subsequent reactivity of the RO+ T cells was unaffected by exposure to EC, indicating a neutral encounter. In contrast, culture with DR+ EC induced allospecific nonresponsiveness in RA+ T cells. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2192539/pdf http://jem.rupress.org/content/183/4/1603.full.pdf |
| ISSN | 00221295 |
| DOI | 10.1084/jem.183.4.1603 |
| Journal | The Journal of experimental medicine |
| Issue Number | 4 |
| Volume Number | 183 |
| Language | English |
| Publisher | Rockefeller University Press |
| Publisher Date | 1996-04-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: The Journal of experimental medicine Research and Experimental Medicine Antigen Presenting Cell Endothelial Cell Major Histocompatibility Complex Immune System |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology |