NDLI: The role of extracellular matrix in the migration and differentiation of parietal endoderm from teratocarcinoma embryoid bodies.

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The role of extracellular matrix in the migration and differentiation of parietal endoderm from teratocarcinoma embryoid bodies.

Content Provider	Scilit
Author	Grabel, L. B. Watts, T. D.
Copyright Year	1987
Description	Embryoid bodies formed from teratocarcinoma stem cells differentiate an outer layer consisting of parietal and visceral endoderm or of visceral endoderm exclusively. We have previously shown that when these embryoid bodies are plated on collagen-coated substrates a parietal endoderm-like cell migrates onto the substrate, whereas all of the visceral endoderm remains associated with the stem cell mass, suggesting a role for substrate contact in parietal endoderm differentiation. We now identify fibronectin as the migration-promoting component in these cultures, and note that laminin and collagen type IV are 10-fold less effective at promoting both attachment and endoderm outgrowth. The RGDS tetrapeptide (arg-gly-asp-ser) from the cell attachment domain of fibronectin can specifically block attachment and outgrowth on both fibronectin- and laminin-coated substrates. In addition, the involvement of the 140-kD fibronectin receptor is demonstrated using an antibody directed against this molecule.
Related Links	http://jcb.rupress.org/content/jcb/105/1/441.full.pdf
Ending Page	448
Page Count	8
Starting Page	441
DOI	10.1083/jcb.105.1.441
Journal	Journal of Cell Biology
Issue Number	1
Volume Number	105
Language	English
Publisher	Rockefeller University Press
Publisher Date	1987-07-01
Access Restriction	Open
Subject Keyword	Developmental Biology Differentiation Parietal Endoderm Embryoid Bodies Attachment Teratocarcinoma Outgrowth Antibody Journal: Journal of Cell Biology (Vol- 105, Issue- 1)
Content Type	Text

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