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Signal transducer and activators of transcription 3 regulates cryptotanshinone-induced apoptosis in human mucoepidermoid carcinoma cells
| Content Provider | Scilit |
|---|---|
| Author | Cho, Sung-Dae Yu, Hyun-Ju Park, Chul Kim, Sun-Ju Cho, Nam-Pyo |
| Copyright Year | 2014 |
| Abstract | Background: Cryptotanshinone (CT) is a biologically active compound from the root of Salvia miltiorrhiza that has been reported to induce apoptosis in various cancer cell lines; but, it has not yet been fully explored in human mucoepidermoid carcinoma (MEC). Objective: Here, we demonstrated the apoptotic effects and its related mechanism in MC-3 and YD-15 human MEC cell lines. Materials and Methods: The effects of CT on apoptotic activity were evaluated by cell proliferation assay, Western blotting, 4'-6-diamidino-2-phenylindole staining, reverse transcription-polymerase chain reaction, and luciferase assay. Results: Our data show that CT treatment of MC-3 cells results in anti-proliferative and apoptotic activities in MC-3 and it is accompanied by a decrease in phosphorylation and dimerization of signal transducer and activators of transcription 3 (STAT3). CT decreased the expression levels of myeloid cell leukemia-1 (Mcl-1) and surviving, whereas Bcl-xL expression was not changed. CT clearly regulates survivin protein at a transcriptional level and alters Mcl-1 through proteasome-dependent protein degradation. In addition, CT-induced apoptotic cell death in YD-15, another human MEC cell line, was associated with the inhibition of STAT3 phosphorylation. Conclusion: These data suggest that CT could be a good apoptotic inducer through modification of STAT3 signaling in human MEC cell lines. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189281/pdf http://www.phcog.com/article.asp?issn=0973-1296;year=2014;volume=10;issue=39;spage=622;epage=629;aulast=Yu;type=2 |
| File Format | XHTML |
| ISSN | 09731296 |
| e-ISSN | 09764062 |
| DOI | 10.4103/0973-1296.139802 |
| Journal | Pharmacognosy Magazine |
| Issue Number | 39 |
| Volume Number | 10 |
| Language | English |
| Publisher | Medknow |
| Publisher Date | 2014-01-01 |
| Access Restriction | Open |
| Subject Keyword | Toxicology Apoptosis Cryptotanshinone Myeloid Cell Leukemia-1 Mucoepidermoid Carcinoma Signal Transducer and Activator of Transcription 3 Survivin Pharmacognosy Magazine, Volume 10, Issue 39 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmaceutical Science |
