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Anti–oxidative and anti–inflammatory effects of Tagetes minuta essential oil in activated macrophages
| Content Provider | Scilit |
|---|---|
| Author | Karimian, Parastoo Kavoosi, Gholamreza Amirghofran, Zahra |
| Copyright Year | 2014 |
| Abstract | To investigate antioxidant and anti-inflammatory effects of Tagetes minuta (T. minuta) essential oil. In the present study T. minuta essential oil was obtained from leaves of T. minuta via hydro-distillation and then was analyzed by gas chromatography-mass spectrometry. The anti-oxidant capacity of T. minuta essential oil was examined by measuring reactive oxygen, reactive nitrogen species and hydrogen peroxide scavenging. The anti-inflammatory activity of T. minuta essential oil was determined through measuring NADH oxidase, inducible nitric oxide synthase and TNF-α mRNA expression in lipopolysacharide-stimulated murine macrophages using real-time PCR. Gas chromatography-mass spectrometry analysis indicated that the main components in the T. minuta essential oil were dihydrotagetone (33.86%), E-ocimene (19.92%), tagetone (16.15%), cis-β-ocimene (7.94%), Z-ocimene (5.27%), limonene (3.1%) and epoxyocimene (2.03%). The T. minuta essential oil had the ability to scavenge all reactive oxygen/reactive nitrogen species radicals with IC50 12-15 µg/mL, which indicated a potent radical scavenging activity. In addition, T. minuta essential oil significantly reduced NADH oxidase, inducible nitric oxide synthaseand TNF-α mRNA expression in the cells at concentrations of 50 µg/mL, indicating a capacity of this product to potentially modulate/diminish immune responses. T. minuta essential oil has radical scavenging and anti-inflammatory activities and could potentially be used as a safe effective source of natural anti-oxidants in therapy against oxidative damage and stress associated with some inflammatory conditions. |
| Related Links | https://core.ac.uk/download/pdf/82240688.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868793/pdf |
| Ending Page | 227 |
| Page Count | 9 |
| Starting Page | 219 |
| File Format | XHTML |
| ISSN | 22211691 |
| DOI | 10.1016/s2221-1691(14)60235-5 |
| Journal | Asian Pacific Journal of Tropical Biomedicine |
| Issue Number | 3 |
| Volume Number | 4 |
| Language | English |
| Publisher | Medknow |
| Access Restriction | Open |
| Subject Keyword | Integrative and Complementary Medicine Anti-inflammatory Antioxidant Essential Oil Macrophages Tagetes Minuta Asian Pacific Journal of Tropical Biomedicine, Volume 4, Issue 3 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
