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Isolation and identification of phenolic compounds from Gynura divaricata leaves
| Content Provider | Scilit |
|---|---|
| Author | Cao, Shuwen Wan, Chunpeng Yu, Yanying Zhou, Shouran Tian, Shuge |
| Copyright Year | 2011 |
| Abstract | Background: Phenolic constituents were the principle bioactivity compounds exist in Gynura divaricata, little phenolic compounds were reported from the plant previously. Materials and Methods: 60% ethanol extract from the leaves of Gynura divaricata were isolated and purified by column chromatography of Silica gel, ODS and Sephadex LH-20, the structures of the isolated compounds were identified by UV, 1H-NMR, 13C-NMR and MS spectroscopic techniques. Additionally, a high-performance liquid chromatography-diode array detector-electrospray ionization-mass (HPLC-DAD-ESI-MS) analytical method was developed to identify some minor constituents in the n-butanol fraction of the ethanol extract of Gynura divaricata. Results: Six flavonols and one Dicaffeoylquinic acid were isolated from the leaves of Gynura divaricata, and these compounds were identified as follows: quercetin (1), kaempferol (2), kaempferol-3-O-β-D-glucopyranoside (3), quercetin-3-O-rutinoside (4), kaempferol-3,7-di-O-β-D-glucopyranoside (5), kaempferol-3-O-rutinoside-7-O-β-D-glucopyranoside (6), and 3,5-dicaffeoylquinic acid (7). A total of 13 compounds, including 9 flavonol glycosides and 4 phenolic acids, were tentatively identified by comparing their retention time (RT), UV, and MS spectrum values with those that had been identified and the published data. Conclusion: This was the first time to use the HPLC-DAD-ESI-MS method to identify the phytochemicals of the genera Gynura. Moreover, compounds (6) and (7) have been isolated for the first time from the genus Gynura. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113347/pdf http://www.phcog.com/article.asp?issn=0973-1296;year=2011;volume=7;issue=26;spage=101;epage=108;aulast=Wan;type=2 |
| File Format | XHTML |
| ISSN | 09731296 |
| e-ISSN | 09764062 |
| DOI | 10.4103/0973-1296.80666 |
| Journal | Pharmacognosy Magazine |
| Issue Number | 26 |
| Volume Number | 7 |
| Language | English |
| Publisher | Medknow |
| Publisher Date | 2011-01-01 |
| Access Restriction | Open |
| Subject Keyword | Agronomy Gynura Divaricata Dc. Hplc-dad-esi-ms Phenolic Constituents Pharmacognosy Magazine, Volume 7, Issue 26 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmaceutical Science |
