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blaCTX-M, blaTEM, and blaSHV in Enterobacteriaceae from North-Indian tertiary hospital: high occurrence of combination genes
| Content Provider | Scilit |
|---|---|
| Author | Singh, Anuradha Sobia, Farrukh Rashid, Mohammad Malik, Abida Shukla, Indu Khan, Haris Manzoor Shahid, Mohammed |
| Copyright Year | 2011 |
| Abstract | To delineate the frequency of occurrence of $bla_{CTX-M}$, $bla_{TEM}$, and $bla_{SHV}$ in Enterobacteriaceae from North-Indian tertiary hospital. A random collection of a subset of 45 Escherichia coli (E. coli) and 28 Klebsiella pneumoniae (K. pneumoniae) that was resistant to a third generation cephalosporin and obtained during 2007–2008 was selected for detailed screening for $bla_{CTX-M}$, $bla_{TEM}$, and $bla_{SHV}$ by monoplex PCRs. The isolates demonstrating the presence of $bla_{CTX-M}$ alleles were characterized for the specific CTX-M-genogroup by using a multiplex PCR. Resistance to cefoperazone, ceftazidime, ceftriaxone, cefotaxime, cefoxitin and piperacillin was 100% each in K. pneumoniae isolates, whereas these resistance-rates for E. coli isolates were 93.1%, 83.8%, 91.9%, 93.6%, 97.3% and 97.1%, respectively. Concomitant resistance to aminoglycosides, quinolones and aztreonam was also noticed. Presence of any of the bla genes $(bla_{CTX-M}$, $bla_{TEM}$, and $bla_{SHV}$) was noticed in a total of 28 (38.4%) isolates of the 73 isolates studied. Many isolates demonstrated occurrence of these genes in various combinations. $bla_{CTX-M}$, $bla_{TEM}$, and $bla_{SHV}$ were noticed in 28.8%, 10.9% and 13.7% isolates, respectively. Multiplex PCR in $bla_{CTX-M}$ harboring isolates demonstrated the presence of CTX-M-Genogroup-1 and sequencing for the specific CTX-M-type revealed presence of CTX-M-15 type. RAPD typing showed wide diversity in isolates. This is amongst the premier report describing the simultaneous occurrence of $bla_{TEM}$, $bla_{SHV}$, and $bla_{ampC}$ in Indian Enterobacteriaceae and that wider dissemination of these genes, as demonstrated by diversity of isolates, raises concern and emphasizes a need for extensive search for the presence of these gene pools in Indian subcontinent. |
| Related Links | https://core.ac.uk/download/pdf/82376516.pdf |
| Ending Page | 105 |
| Page Count | 5 |
| Starting Page | 101 |
| File Format | XHTML |
| ISSN | 19957645 |
| e-ISSN | 23524146 |
| DOI | 10.1016/s1995-7645(11)60046-1 |
| Journal | Asian Pacific Journal of Tropical Medicine |
| Issue Number | 2 |
| Volume Number | 4 |
| Language | English |
| Publisher | Medknow |
| Access Restriction | Open |
| Subject Keyword | Gastroenterology and Hepatology Resistance Genes Blactx-m-15 Blatem Blashv Concomitant-occurrence Enterobacteriaceae Asian Pacific Journal of Tropical Medicine, Volume 4, Issue 2 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
