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Potential lipid-lowering effects ofEleusine indica(L) Gaertn. Extract on high-fat-diet-induced hyperlipidemic rats
| Content Provider | Scilit |
|---|---|
| Author | Lai, How Yee Ong, Siew Ling Nalamolu, Koteswara Rao |
| Copyright Year | 2017 |
| Abstract | Background: To date, anti-obesity agents based on natural products are tested for their potential using lipase inhibition assay through the interference of hydrolysis of fat by lipase resulting in reduced fat absorption without altering the central mechanisms. Previous screening study had indicated strong anti-obesity potential in Eleusine indica (E. indica), but to date, no pharmacologic studies have been reported so far. Objective: This study was performed to investigate the lipid-lowering effects of E. indica using both in vitro and in vivo models. Methods: The crude methanolic extract of E. indica was fractionated using hexane (H-Ei), dichloromethane (DCM-Ei), ethyl acetate (EA-Ei), butanol (B-Ei), and water (W-Ei). All the extracts were tested for antilipase activity using porcine pancreatic lipase. Because H-Ei showed the highest inhibition, it was further subjected to chemical profiling using high-performance liquid chromatography. Subsequently, oral toxicity analysis of H-Ei was performed [Organization for Economic Cooperation and Development guidelines using fixed dose procedure (No. 420)]; efficacy analysis was performed using high-fat diet (HFD)-induced hyperlipidemic female Sprague–Dawley rats. Results: According to the toxicity and efficacy analyses, H-Ei did not demonstrate any noticeable biochemical toxicity or physiologic abnormalities and did not cause any tissue damage as per histologic analysis. Furthermore, H-Ei significantly reduced body weight and improved serum profile and did not show hepatotoxicity and nephrotoxicity based on the serum profile. Moreover, H-Ei alleviated HFD-induced hepatosteatosis and ameliorated induced adiposity in both visceral and subcutaneous adipose tissue. Conclusion: Our results demonstrate that H-Ei effectively improved hyperlipidemia. Further studies to explore its possibility as an alternative pharmacologic agent to treat obesity are warranted. Abbreviation Used: ALT: Alanine transaminase; AST: Aspartate transaminase; B-Ei: Butanol extract of E. indica; DCM-Ei: Dichloromethane extract of E. indica; EA-Ei: Ethyl acetate extract of E. indica; GHS: Globally Harmonized System; HDL: High-density lipoprotein; H-Ei: Hexane extract of E. indica; HFD: High-fat diet; HPLC: High-performance liquid chromatography; LDL: Low-density lipoprotein; NFD: Normal fed diet; PPL: Porcine pancreatic lipase; SEM: Standard error of mean; SD: Standard deviation; TC: Total cholesterol; TG: Triglycerides; W-Ei: Water extract of E. indica. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5407099/pdf http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=49;spage=1;epage=9;aulast=Ong;type=2 |
| File Format | XHTML |
| ISSN | 09731296 |
| e-ISSN | 09764062 |
| DOI | 10.4103/0973-1296.203986 |
| Journal | Pharmacognosy Magazine |
| Issue Number | 49 |
| Volume Number | 13 |
| Language | English |
| Publisher | Medknow |
| Access Restriction | Open |
| Subject Keyword | Integrative and Complementary Medicine Toxicology Anti-obesity Eleusine Indica High-fat Diet Hyperlipidemia Lipase Inhibition Toxicity Pharmacognosy Magazine, Volume 13, Issue 49 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmaceutical Science |
