NDLI: Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells

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Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells

Content Provider	Scilit
Author	Mat, Nor Fazila Che Wahab, Nurul Hidayah Din, Nur Afina Mohd Lim, Yee Ying Jamil, Noor Izani Noor
Copyright Year	2019
Abstract	Objective: To examine the proapoptotic properties of Oroxylum indicum methanol extract on cervical cancer cells. Methods: Methylene blue assay was used to determine the $IC_{50}$ value of the extract. Western blotting assays were done to analyze the expression of HPV oncoproteins (HPV18 E6 and E7) and apoptotic molecules (caspase-3 and caspase-8). Reverse transcriptase PCR assays were performed to determine genetic alteration of tumor suppressors p53 and pRb and apoptosis markers Fas and FasL. Enzyme-linked immunosorbent assay (ELISA) was done to determine the expression of cytokine levels (IL-6 and IL-12). Results: The determination of $IC_{50}$ value indicated a higher anti-proliferative activity of the extract compared to cisplatin. After 24 hours of treatment, Western blot analysis showed that treated HeLa cells exhibited a significant down-regulation of HPV18 oncoproteins E6 and E7, and a significant induction of caspase-8 and caspase-3 activation level. Meanwhile, the mRNA expressions of p53, pRb, Fas and FasL were significantly upregulated in treated cells. Moreover, ELISA showed an increased IL-12 and decreased IL-6 production after Oroxylum indicum treatment. Conclusions: The methanol extract of Oroxylum indicum has an anti-proliferative activity and proapoptotic potential. It induces localized-immunity improvements by altering cytokine production in HPV-positive cervical cancer cells.
Related Links	https://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=8;spage=339;epage=345;aulast=Wahab;type=2
File Format	XHTML
ISSN	22211691
DOI	10.4103/2221-1691.262080
Journal	Asian Pacific Journal of Tropical Biomedicine
Issue Number	8
Volume Number	9
Language	English
Publisher	Medknow
Access Restriction	Open
Subject Keyword	Integrative and Complementary Medicine Oroxylum Indicum Proapoptotic Anti-proliferative Hela Cells Asian Pacific Journal of Tropical Biomedicine, Volume 9, Issue 8
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

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