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Effects of leukocyte–platelet-rich fibrin and advanced platelet-rich fibrin on the viability and migration of human gingival fibroblasts
| Content Provider | Scilit |
|---|---|
| Author | Shokri, Maryam Esfahrood, Zeinab Rezaei Ardakani, Mohammadreza Talebi Shokri, Mehdi |
| Copyright Year | 2020 |
| Abstract | Background: Platelet products play a fundamental role in the process of healing. The new generation of platelet-rich fibrin (PRF), namely advanced PRF (A-PRF), has different biological and mechanical properties compared to those of leukocyte-PRF (L-PRF). This study aimed to compare the effects of L-PRF and A-PRF on the viability and migration of human gingival fibroblasts (HGFs). Materials and Methods: In this in vitro study, the effects of A-PRF and L-PRF on the viability and migration of HGFs after 24 and 48 h were evaluated using the methyl thiazolyl tetrazolium assay. The viability of the negative control culture medium was considered to be 100%. The mean optical density of the test groups was divided by that of the negative control group and reported as percentage. One-way ANOVA was applied to assess the effects of time and type of PRF on the viability and migration of HGFs. Pairwise comparisons were made using the Tukey's test. Results: At 24 h, cell viability in the L-PRF group was significantly higher than that in the A-PRF group (P < 0.05). However, no significant difference was noted between the two groups at 48 h. At 24 h, L-PRF caused significantly higher cell migration compared to the negative control group, whereas at 48 h, both A-PRF and L-PRF significantly increased cell migration compared to the control group. Conclusion: Within the limitations of this study, L-PRF and A-PRF had significant effects on the viability and migration of HGFs. Further studies on these platelet concentrates are warranted. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961444/pdf https://www.jisponline.com/article.asp?issn=0972-124X;year=2020;volume=24;issue=1;spage=15;epage=19;aulast=Esfahrood;type=2 |
| Ending Page | 19 |
| Page Count | 5 |
| Starting Page | 15 |
| File Format | XHTML |
| ISSN | 0972124X |
| e-ISSN | 09751580 |
| DOI | 10.4103/jisp.jisp_218_19 |
| Journal | Journal of Indian Society of Periodontology |
| Issue Number | 1 |
| Volume Number | 24 |
| Language | English |
| Publisher | Medknow |
| Publisher Date | 2020-01-01 |
| Access Restriction | Open |
| Subject Keyword | Dentistry and Oral Surgery Cell Migration Cell Viability Fibroblast Platelet-rich Fibrin Journal of Indian Society of Periodontology, Volume 24, Issue 1 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Periodontics |
