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Inhibition of wheat bran and it′s active compoments on α-glucosidase in vitro
| Content Provider | Scilit |
|---|---|
| Author | Chen, Jun Zhu, Shuyun Zhang, Chunxiao Chen, Hua Liu, Youbing Tu, Jie |
| Copyright Year | 2013 |
| Abstract | Background: Wheat bran is a traditional Chinese medicine; however, it is mostly used as feedstuff in China. Wheat bran is widely accepted as an important ingredient in many low-glycemic index foods in modern western societies; however, its glycemic control mechanism is unknown. Objective: To determine potent α-glucosidase inhibitory compounds from wheat bran and to identify the inhibition on α-glucosidase . Materials and Methods: Ethanolic extract of wheat bran was prepared to evaluate the inhibitory activity on α-glucosidase, then fractionation of the extract was guided by in vitro enzyme-inhibition assay, and the potent α-glucosidase inhibitory compounds were identified by high performance liquid chromatography and atmospheric pressure chemical ionization-mass spectrometry; finally the enzyme inhibition process was studied using the Michaelis-Menton and the Lineweaver-Burk equations. Results: Both baker's yeast and rat intestinal enzymes were mostly inhibited (87.9% and 66.8% inhibition, respectively) at concentration 0.6 mg/mL of the ethanolic extract of wheat bran. The petroleum ether fraction in the ethanolic extract of wheat bran showed significant activity against rat intestinal α-glucosidase, and revealed a dose-dependent effect. The inhibition was 76.57% at 0.3 mg/mL and 100% at 0.6 mg/mL. The active fraction 13 of petroleum ether fraction was identified as alkylresorcinols (ARs). ARs showed strong inhibition towards α-glucosidase and its IC$ _{50}$ value was found to be 37.58 ΅g/mL. The enzyme kinetic studies showed that, in the presence of ARs, the Michaelis-Menton constant $(K_{m}$ ) remains constant whereas the maximal velocity $(V_{max}$ ) decreases, revealing a non-competitive type of inhibition. Conclusion: The therapeutic potentiality of ARs in the management of the postprandial hyperglycemia will proliferate the utilization of wheat bran in controlling type 2 diabetes. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3793335/pdf http://www.phcog.com/article.asp?issn=0973-1296;year=2013;volume=9;issue=36;spage=309;epage=314;aulast=Tu;type=2 |
| File Format | XHTML |
| ISSN | 09731296 |
| e-ISSN | 09764062 |
| DOI | 10.4103/0973-1296.117826 |
| Journal | Pharmacognosy Magazine |
| Issue Number | 36 |
| Volume Number | 9 |
| Language | English |
| Publisher | Medknow |
| Access Restriction | Open |
| Subject Keyword | Integrative and Complementary Medicine Alkylresorcinols Inhibition Wheat Bran Α-glucosidase Pharmacognosy Magazine, Volume 9, Issue 36 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmaceutical Science |
