Loading...
Please wait, while we are loading the content...
Elucidation of molecular mechanism(s) of cognition enhancing activity of $Bacomind^{®}$: A standardized extract of bacopa monnieri
| Content Provider | Scilit |
|---|---|
| Author | Dethe, Shekhar Deepak, M. Agarwal, Amit |
| Copyright Year | 2016 |
| Abstract | Background: Bacopa monnieri (L.) Wettst., commonly known as Brahmi, is renowned in Indian traditional system for its potent memory enhancing activity, which has been validated by various scientific studies. Objective: The objective of this study was to understand the molecular mechanism of memory enhancing activity of $BacoMind^{®}$ (BM), a standardized extract of B. monnieri. Materials and Methods: BM was screened in vitro in a panel of cell-free and receptor-transfected cell assays. The purified enzymes/membrane homogenates/cells were incubated with substrate/standard ligand in the absence or presence of the test compound. The IC$ _{50}$ values and EC$ _{50}$ values were determined by nonlinear regression analysis of the concentration-response curves generated with mean replicate values using Hill equation curve fitting. Results: BM was found to inhibit three enzymes; Catechol-O-methyl transferase (COMT), Prolyl endopeptidase (PEP), and Poly (ADP-ribose) polymerase (PARP). It also had an antagonistic effect on serotonin 6 and 2A (5-HT$ _{6}$ and 5-HT$ _{2A}$ ) receptors$ _{,}$ known to influence the different neurological pathways, associated with memory and learning disorders, age-associated memory impairment. Conclusion: BM was found to inhibit three enzymes namely, Catechol-O-methyl transferase (COMT), Prolyl endopeptidase (PEP), and Poly (ADP-ribose) polymerase (PARP). It also exhibited an antagonistic effect on 5-HT$ _{6}$ and 5-HT$ _{2A}$ receptors. Abbreviations used: HTRF: Homogenous time resolved fluorescence, cAMP: Cyclic adenosine monophosphate, CHO : Chinese hamster ovary, RFU : Relative fluorescence unit, pNP: Para nitro phenol, AMC: 7-amino-4-methylcoumarin, ELISA: Enzyme linked immunosorbent assay, Z-Pro-Pro-CHO: Z-prolyl-prolinal, HEK: Human embryonic kidney, TE: Trolox equivalent. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068128/pdf http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=47;spage=482;epage=487;aulast=Dethe;type=2 |
| File Format | XHTML |
| ISSN | 09731296 |
| e-ISSN | 09764062 |
| DOI | 10.4103/0973-1296.191464 |
| Journal | Pharmacognosy Magazine |
| Issue Number | 47 |
| Volume Number | 12 |
| Language | English |
| Publisher | Medknow |
| Access Restriction | Open |
| Subject Keyword | Integrative and Complementary Medicine Bacopa Monnieri Bacomind Cognition Enzyme Inhibition Learning and Memory Mechanism of Action Serotonin Receptors Pharmacognosy Magazine, Volume 12, Issue 47 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmaceutical Science |
