The efficacy of okra fruit extract on the expression of transforming growth factor beta 1 in the tooth socket of diabetic Wistar rats

Content Provider	Scilit
Author	Luthfi, Muhammad Wijayanti, Elvina Hasna Razak, Fathilah Binti Abdul Irmalia, Wahyuning Ratih Yuliati
Copyright Year	2021
Abstract	Background: Patients with diabetes mellitus suffer from an additional macrophage dysfunction in the secretion of growth factor, which later decreases transforming growth factor beta 1 (TGF-β1). This condition disrupts proliferation and angiogenesis. Extract of okra fruit (Abelmoschus esculentus) contains flavonoid, an active substance which acts as antioxidant, anti-inflammation, and antidiabetes. The purpose of this study is to analyze the difference in TGF-β1 expression in wound-healing process after tooth extraction of diabetic Wistar rats. Materials and Methods: This is a laboratory experimental study using pretest and posttest on 24 Wistar rats which are divided into two groups: control group (treated with streptozotocin induction but without administration of okra fruit extract) and treatment group (treated with streptozotocin induction and oral administration of 250 mg/kg okra fruit extract once a day). Extractions of the rats' mandibular left incisors were performed using a pair of modified forceps and an elevator. The tooth sockets were then irrigated using saline solution. Four rats in each group were sacrificed on day 3 (KO1, PO1), 5 (KO2, PO2), and 7 (KO3, PO3). The socket tissues from the rats were then immunohistochemically analyzed. Data were analyzed at level significance of 0.05. Results: The average level of TGF-β1 expression in the treatment groups was higher compared to the control group: PO1 (11.59 ± 0.58), PO2 (15.15 ± 1.07), and PO3 (18.75 ± 2.73) as compared to KO1 (5.32 ± 1.69), KO2 (8.47 ± 0.60), and KO3 (9.28 ± 1.16) with P = 0.001. Conclusion: The administration of okra fruit extract can increase the level of TGF-β1 in wounds after tooth extraction of diabetic Wistar rats.
Related Links	https://www.drjjournal.net/article.asp?issn=1735-3327;year=2021;volume=18;issue=1;spage=91;epage=91;aulast=Luthfi;type=2
File Format	XHTML
ISSN	17353327
e-ISSN	20080255
DOI	10.4103/1735-3327.330872
Journal	Dental Research Journal
Issue Number	1
Volume Number	18
Language	English
Publisher	Medknow
Access Restriction	Open
Subject Keyword	Integrative and Complementary Medicine Diabetes Mellitus Okra Fruit Transforming Growth Factor Beta 1 Wound Healing Dental Research Journal, Volume 18, Issue 1
Content Type	Text
Resource Type	Article
Subject	Dentistry