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Antithrombocytopenic potential of bioactivity guided fractions of traditionally used Psidium guajava Linn. leaves in busulfan induced-thrombocytopenic rats
| Content Provider | Scilit |
|---|---|
| Author | Ahmad, Sayeed Ahmad, Adil Ibrahim, Mohammad Chester, Karishma Khan, Washim Ansari, Sh |
| Copyright Year | 2019 |
| Abstract | Background: Psidium guajava L. (family: Myrtaceae) is a traditionally used medicinal plant and possesses various therapeutic properties. This study was aimed to explore the antithrombocytopenic potential of bioactive fraction of P. guajava L. Materials and Methodology: Hydroalcoholic extract of dried leaves was prepared through soxhlation, and further, it was fractionated. Hydroalcoholic extract and its fractions were tested for antithrombocytopenic potential in busulfan-treated rats. Bioactive fractions were metabolically characterized through ultra-performance liquid chromatography–mass spectrometry (UPLC-MS) and. Results: A total of 30.3% w/w hydroalcoholic extract was obtained from which 5.7%, 4.2%, 8.6%, and 11.8% w/w of n-hexane, dichloromethane, n-butanol, and aqueous fractions were collected, respectively. n-butanol and aqueous fractions were identified as the potent bioactive fractions as antithrombocytopenic agents. Using toluene:ethyl acetate:formic acid (6:3:1 v/v/v) as a solvent system in TLC, 12 and 9 metabolites were separated in n-butanol and aqueous fractions, respectively, whereas 11 and 12 metabolites were tentatively identified through UPLC-MS in aqueous and n-butanol fractions, respectively. Five metabolites (showing m/z values of 303.05, 302.5, 313.2, 573.51, and 341.32 eluted at 2.80, 3.81, 7.94, 8.55 and 8.69 min, respectively) were found in both aqueous and n-butanol fractions. Conclusion: Chromatographically characterized n-butanol and aqueous fractions of hydroalcoholic extract of P. guajava increased platelet count, and it can be further explored for the development of new phytopharmaceutical. |
| Related Links | http://www.phcog.com/article.asp?issn=0973-1296;year=2019;volume=15;issue=66;spage=440;epage=448;aulast=Ahmad;type=2 |
| File Format | XHTML |
| ISSN | 09731296 |
| e-ISSN | 09764062 |
| DOI | 10.4103/pm.pm_608_18 |
| Journal | Pharmacognosy Magazine |
| Issue Number | 66 |
| Volume Number | 15 |
| Language | English |
| Publisher | Medknow |
| Publisher Date | 2019-01-01 |
| Access Restriction | Open |
| Subject Keyword | Integrative and Complementary Medicine Antithrombocytopenic Activity Guava Leaves Psidium Guajava Thin-layer Chromatography Ultra-performance Liquid Chromatography–mass Spectrometry Pharmacognosy Magazine, Volume 15, Issue 66 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmaceutical Science |
