NDLI: Implication of a positive virtual crossmatch with negative flow crossmatch: A mind-boggler

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Implication of a positive virtual crossmatch with negative flow crossmatch: A mind-boggler

Content Provider	Scilit
Author	Agrawal, Soma Chowdhry, Mohit Thakur, Yogita Guleria, Sandeep Sharma, Vandana
Copyright Year	2020
Abstract	There are occasions when tests performed before considering a patient for transplant are ambiguous and require further workup. One such condition is the presence of a positive virtual crossmatch (VXm) (anti-human leukocyte antigen [HLA-A]26: 01 antibody in this case) with a negative complement-dependent cytotoxicity, Luminex, and flow crossmatch. To ascertain the nature of the antibody, the beads used in single-antigen bead assay (SAB) were treated by acid to denature the antigens and retested with the control and test sample. The mean fluorescence intensities (MFIs) from the patient sera with acid-treated beads increased considerably as compared to the regularly untreated SAB indicating additional antigen epitopes become available by the denaturation process. The MFIs of the antibodies from that of the control sera were reduced to half on testing with the acid-treated SAB assay, indicating that HLA antigen HLA-A26 was susceptible to acid treatment. Therefore, results of VXm should be interpreted with caution.
Related Links	http://www.ajts.org/article.asp?issn=0973-6247;year=2020;volume=14;issue=1;spage=79;epage=82;aulast=Chowdhry;type=2
Ending Page	82
Page Count	4
Starting Page	79
File Format	XHTML
ISSN	09736247
e-ISSN	19983565
DOI	10.4103/ajts.ajts_159_18
Journal	Asian Journal of Transfusion Science
Issue Number	1
Volume Number	14
Language	English
Publisher	Medknow
Publisher Date	2020-01-01
Access Restriction	Open
Subject Keyword	Medical Laboratory Technology False-positive Single Antigen Bead Assay Renal Transplant Transplant Immunology Asian Journal of Transfusion Science, Volume 14, Issue 1
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Hematology

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