Quantification analysis and In vitro anti-inflammatory effects of 20-hydroxyecdysone, momordin ic, and oleanolic acid from the fructus of Kochia scoparia

Content Provider	Scilit
Author	Seo, Chang-Seob Yoo, Sae-Rom Jeong, Soo-Jin Lee, Na-Ri Shin, Hyeun-Kyoo
Copyright Year	2017
Abstract	Background: The fructus of Kochia scoparia Schrader (Chenopodiaceae) is a traditional herbal medicine that has been used for treating gonorrhea and dermatitis. Objective: We investigated the anti-inflammatory activities of three marker compounds, including 20-hydroxyecdysone, momordin Ic, and oleanolic acid, from the fructus of K. scoparia. Materials and Methods: The simultaneous analysis of three components was performed using high-performance liquid chromatography and high-performance thin-layer chromatography. We evaluated the anti-inflammatory effects of the nine marker compounds by determining their anti-inflammatory activities in the murine macrophage cell line RAW 264.7. Results: Among three marker compounds, momordin Ic, but not 20-hydroxyecdysone and oleanolic acid, had inhibitory effects on the production of inflammatory cytokines tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in LPS-treated RAW264.7 macrophages. The effects of three marker compounds on prostaglandin $E_{2}(PGE_{2}$) were also evaluated. All three compounds significantly reduced $PGE_{2}$production in LPS-treated cells. Conclusions: We suggest that momordin Ic is the most potent phytochemical of the fructus of K. scoparia as an anti-inflammatory agent. Abbreviations used: HPLC: High-performance liquid chromatography; TNF-α: Tumor necrosis factor alpha; IL-6: Interleukin-6; $PGE^{2}$: Pro-inflammatory mediator prostaglandin $E_{2}$; LPS: Lipopolysaccharide.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551347/pdf http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=51;spage=339;epage=344;aulast=Yoo;type=2
Ending Page	344
Page Count	6
Starting Page	339
File Format	XHTML
ISSN	09731296
e-ISSN	09764062
DOI	10.4103/0973-1296.211023
Journal	Pharmacognosy Magazine
Issue Number	51
Volume Number	13
Language	English
Publisher	Medknow
Access Restriction	Open
Subject Keyword	Integrative and Complementary Medicine Anti-inflammatory Effect Kochia Scoparia 20-hydroxyecdysone Momordin Ic Oleanolic Acid Raw264.7 Pharmacognosy Magazine, Volume 13, Issue 51
Content Type	Text
Resource Type	Article
Subject	Drug Discovery Pharmaceutical Science