Optimization and molecular characterization of novel Aspergillus spp. producing invertase enzyme

Content Provider	Scilit
Author	Salim, Rasha G. Khalil, Bigad E. Abo-Sereh, Nivien A.
Copyright Year	2020
Abstract	Background and objective Invertase, one of the most important enzymes used in industrial and pharmaceutical applications, has been produced from different Aspergillus spp. Statistical and mathematical tools [response surface methodology (RSM)] have been carried out to optimize culture conditions to facilitate maximum enzyme production. This investigation aims to screen the ability of some Aspergillus spp. to produce invertase enzyme, to optimize the culture parameters, and to detect the genetic diversity between them. Materials and methods A total of five fungal strains were tested for the production of invertase. The most promising strain was selected for optimization. Optimization experiments were carried out to obtain maximum enzyme production, and a molecular marker (RAPD-PCR) was used to differentiate between these strains. Results and conclusion Some Aspergillus spp. were examined to produced invertase enzyme after 2 days of cultivation. Aspergillus niger recorded the highest enzyme production (6.8 U/ml). Ten RAPD-PCR primers were used to differentiate between Aspergillus spp. A total of 131 bands were amplified in the five strains of Aspergillus spp. Based on RAPD-PCR analysis, the highest similarities obtained between A. parasiticus and A. niger showed 75%. Moreover, the RAPD-PCR technique is a good tool for differentiation between the Aspergillus spp. To optimize culture condition, RSM was used to increase invertase production by A. niger. Temperature, pH, and incubation time dramatically affect invertase yield. A combination of these factors (pH 5, 35°C, and incubation for 72 h) was optimum for maximum production of invertase (8.2 U/ml). Our results employed RSM to elucidate the combination of effective factors such as temperature, pH, and incubation time to obtain a high yield of an important enzyme, invertase, from A. niger, used as a promising candidate for industrial applications.
Related Links	http://www.epj.eg.net/article.asp?issn=1687-4315;year=2020;volume=19;issue=4;spage=321;epage=329;aulast=Salim;type=2
File Format	XHTML
ISSN	16874315
DOI	10.4103/epj.epj_34_20
Journal	Egyptian Pharmaceutical Journal
Issue Number	4
Volume Number	19
Language	English
Publisher	Medknow
Publisher Date	2020-01-01
Access Restriction	Open
Subject Keyword	Mycology Aspergillus Spp Invertase Optimization and Molecular Characterization Rapd-pcr Response Surface Methodology Egyptian Pharmaceutical Journal, Volume 19, Issue 4
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology Toxicology Applied Microbiology and Biotechnology Pharmaceutical Science