Catechin isolated from Garcinia celebica leaves inhibit Plasmodium falciparum growth through the induction of oxidative stress

Content Provider	Scilit
Author	Abdulah, Rizky Suradji, Eka W. Supratman, Unang Sugijanto, Milyadi Diantini, Ajeng Lestari, Keri Kawazu, Shinichiro Koyama, Hiroshi Subarnas, Anas Barliana, Melisa I.
Copyright Year	2017
Abstract	Background: Resistance of antimalarial drugs to Plasmodium falciparum has become a major concern in malaria eradication. Although it is also affected by several socioeconomic factors, a new antiplasmodial agent is needed for a global malaria control program. Objective: In this study, we attempted to uncover the antiplasmodial properties of Garcinia celebica, an Indonesian medicinal plant, along with the responsible compound and its possible mechanism. Materials and Methods: The G. celebica leaves were ethanol extracted and fractionated based on their polarity using n-hexane, ethyl acetate, and water. The antiplasmodial activity was tested in vitro against chloroquine-resistant P. falciparum at 100 μg/ml for 72 h. The active compound of the most active ethyl acetate fraction was subsequently isolated using column chromatography and identified by nuclear magnetic resonance. Results: The $IC_{50}$of (+)-catechin, the characterized compound, against P. falciparum was 198 μM in 24 h and experiment. The isolated catechin inhibited P. falciparum growth in both trophozoite and schizont stages. An additional experiment also suggests that the antiplasmodial property of catechin occurs through the induction of the oxidative stress to P. falciparum. Conclusion: This result shows that the potential of catechin and its antimalarial properties should be explored further. Abbreviations used: RBC: Red Blood Cells; IC50: Inhibition Concentrattino 50; MeOH: Methanol; RPMI: Roswell Park Memorial Institute; EI: Electron Ionization.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5538170/pdf http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=50;spage=301;epage=305;aulast=Abdulah;type=2
File Format	XHTML
ISSN	09731296
e-ISSN	09764062
DOI	10.4103/pm.pm_571_16
Journal	Pharmacognosy Magazine
Issue Number	50
Volume Number	13
Language	English
Publisher	Medknow
Access Restriction	Open
Subject Keyword	Integrative and Complementary Medicine Catechin Garcinia Celebica Malaria Plasmodium Falciparum Pharmacognosy Magazine, Volume 13, Issue 50
Content Type	Text
Resource Type	Article
Subject	Drug Discovery Pharmaceutical Science