Detection of large deletion in human BRCA1 gene in human breast carcinoma MCF-7 cells by using DNA-Silver Nanoclusters

Content Provider	Scilit
Author	Borghei, Yasaman-Sadat Hosseini, Morteza Ganjali, Mohammad Reza
Copyright Year	2017
Description	Journal: Methods and Applications in Fluorescence Here we describe a label-free detection strategy for large deletion mutation in breast cancer (BC) related gene BRCA1 based on a DNA-silver nanocluster (NC) fluorescence upon recognition-induced hybridization. The specific hybridization of DNA templated silver NCs fluorescent probe to target DNAs can act as effective templates for enhancement of AgNCs fluorescence, which can be used to distinguish the deletion of BRCA1 due to different fluorescence intensities. Under the optimal conditions, the fluorescence intensity of the DNA-AgNCs at emission peaks around 440 nm (upon excitation at 350 nm) increased with the increasing deletion type within a dynamic range from 1.0 × $10^{−10}$ to 2.4 × $10^{−6}$ M with a detection limit (LOD) of 6.4 × $10^{−11}$ M. In this sensing system, the normal type shows no significant fluorescence; on the other hand, the deletion type emits higher fluorescence than normal type. Using this nanobiosensor, we successfully determined mutation using the non-amplified genomic DNAs that were isolated from the BC cell line.
Related Links	http://iopscience.iop.org/article/10.1088/2050-6120/aa8988/ampdf http://iopscience.iop.org/article/10.1088/2050-6120/aa8988/pdf
e-ISSN	20506120
DOI	10.1088/2050-6120/aa8988
Journal	Methods and Applications in Fluorescence
Issue Number	1
Volume Number	6
Language	English
Publisher	IOP Publishing
Publisher Date	2017-12-13
Access Restriction	Open
Subject Keyword	Journal: Methods and Applications in Fluorescence Analytical Chemistry Dna-silver Nanocluster
Content Type	Text
Resource Type	Article