NDLI: Intracellular localization of gold nanoparticles with targeted delivery in MT-4 lymphocytes

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Intracellular localization of gold nanoparticles with targeted delivery in MT-4 lymphocytes

Content Provider	Scilit
Author	Singh, Lavanya Parboosing, Raveen Kruger, Hendrik Gerhardus Maguire, Glenn Govender, Thavendran
Copyright Year	2016
Description	Journal: Advances in Natural Sciences: Nanoscience and Nanotechnology The clinical utility of important therapeutic agents is often limited by the poor permeability of biological membranes. Cell penetrating peptides are usually employed to circumvent this challenge. This approach, coupled with gold nanoparticles, are a promising vehicle for drug delivery due to its good biocompatibility profile, negligable toxicity and possibility for multi-functionalization. Here we report the functionalization and intracellular tracking of gold nanoparticles decorated with a TAT cell penetrating peptide and a fluorescein tag in a simple, two step process. Fluorescence microscopy has confirmed the localization of the functionalized nanoparticles to be inside the cells, specifically within, or in close proximity to the nuclei of MT-4 lymphocytes; a HIV-relevant cell line in which this has not been previously demonstrated. The results of this study demonstrate that TAT has been efficiently conjugated to gold nanoparticles to facilitate both cellular and targeted nuclear entry.
Related Links	http://iopscience.iop.org/article/10.1088/2043-6262/7/4/045013/pdf
ISSN	20436254
e-ISSN	20436262
DOI	10.1088/2043-6262/7/4/045013
Journal	Advances in Natural Sciences: Nanoscience and Nanotechnology
Issue Number	4
Volume Number	7
Language	English
Publisher	IOP Publishing
Publisher Date	2016-10-14
Access Restriction	Open
Subject Keyword	Journal: Advances in Natural Sciences: Nanoscience and Nanotechnology Gold Nanoparticles Therapeutic Agents Fluorescence Microscopy
Content Type	Text
Resource Type	Article

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