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The precise measurement of the fluorescence polarisation anisotropy by single-photon counting
| Content Provider | Scilit |
|---|---|
| Author | Tschanz, H. P. Binker, Th |
| Copyright Year | 1976 |
| Description | Journal: Journal of Physics E: Scientific Instruments The rotational diffusion coefficients of a fluorescent molecule can be determined by measuring the time-dependent anisotropy r(t). The errors introduced in such measurements by misalignments in the setup are discussed. Since the single-photon sampling technique normally used is nonlinear, the necessary normalisation of the intensities I/sub /// and $I_{perpendicular to }$ is considerably complicated. A correct normalisation procedure is described and the sensitivity of the method is shown in a measurement of micellar rotation. |
| Related Links | http://iopscience.iop.org/article/10.1088/0022-3735/9/12/032/pdf |
| Ending Page | 1136 |
| Page Count | 6 |
| Starting Page | 1131 |
| ISSN | 00223735 |
| DOI | 10.1088/0022-3735/9/12/032 |
| Journal | Journal of Physics E: Scientific Instruments |
| Issue Number | 12 |
| Volume Number | 9 |
| Language | English |
| Publisher | IOP Publishing |
| Publisher Date | 1976-12-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Physics E: Scientific Instruments Sampling Technique Diffusion Coefficient |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physics and Astronomy Instrumentation Engineering Materials Science |
