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High-throughput fabrication of vascularized spheroids for bioprinting
| Content Provider | Scilit |
|---|---|
| Author | Moor, Lise De Merovci, Idriz Baetens, Sarah Verstraeten, Julien Kowalska, Paulina Krysko, Dmitri V. Vos, Winnok H. De Declercq, Heidi |
| Copyright Year | 2018 |
| Description | Journal: Biofabrication Overcoming the problem of vascularization remains the main challenge in the field of tissue engineering. As three-dimensional (3D) bioprinting is the rising technique for the fabrication of large tissue constructs, small prevascularized building blocks were generated that can be incorporated throughout a printed construct, answering the need for a microvasculature within the small micron range (< 10 µm). Uniform spheroids with an ideal geometry and diameter for bioprinting were formed, using a high-throughput non-adhesive agarose microwell system. Since monoculture spheroids of endothelial cells were unable to remain stable, coculture spheroids combining endothelial cells with fibroblasts and/or adipose tissue derived mesenchymal stem cells (ADSC) as supporting cells, were created. When applying the favourable coculture ratio, viable spheroids were obtained and endothelial cells spontaneously formed a capillary like network and lumina, as shown by immunohistochemistry and transmission electron microscopy. Especially the presence of ADSC led to a higher vascularization and extracellular matrix (ECM) production of the microtissue. Moreover, spheroids were able to assemble at random in suspension and in a hydrogel, creating a macrotissue. During at random assembly, cells reorganized, creating a branched capillary network throughout the entire fused construct by inoculating with capillaries of adjacent spheroids. Combining the advantage of this natural capacity of microtissues to self-assemble and the controlled organization by bioprinting technologies, these prevascularized spheroids can be useful as building blocks for the engineering of large vascularized 3D tissues. |
| Related Links | http://iopscience.iop.org/article/10.1088/1758-5090/aac7e6/pdf |
| ISSN | 17585082 |
| e-ISSN | 17585090 |
| DOI | 10.1088/1758-5090/aac7e6 |
| Journal | Biofabrication |
| Issue Number | 3 |
| Volume Number | 10 |
| Language | English |
| Publisher | IOP Publishing |
| Publisher Date | 2018-05-25 |
| Access Restriction | Open |
| Subject Keyword | Journal: Biofabrication |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biomaterials Biochemistry Bioengineering Biomedical Engineering Biotechnology |
