NDLI: Simultaneous intraluminal imaging of tissue autofluorescence and eGFP-labeled cells in engineered vascular grafts inside a bioreactor

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Simultaneous intraluminal imaging of tissue autofluorescence and eGFP-labeled cells in engineered vascular grafts inside a bioreactor

Content Provider	Scilit
Author	Li, Cai Alfonso-Garcia, Alba McMasters, James Bec, Julien Weyers, Brent Uyesaka, Lauren Griffiths, Leigh Panitch, Alyssa Marcu, Laura
Copyright Year	2019
Description	Journal: Methods and Applications in Fluorescence The growing demand for tissue engineered vascular grafts (TEVG) motivates the development of optimized fabrication and monitoring procedures. Bioreactors which provide physiologically-relevant conditions are important for improving holistic TEVG properties and performance. Herein we describe a fiber-based intraluminal imaging system that allows for in situ assessment of vascular materials and re-cellularization processes inside a bioreactor by simultaneous and co-registered measurements of endogenous fluorescence lifetime and exogenous marker fluorescence intensity. The lumen of 6 vascular grafts (~4 mm diameter) were scanned by reciprocally rotating a 41° angle polished multimode optical fiber inside a protective glass tube with outer diameter of 3 mm. Tubular bovine pericardium constructs were recellularized using enhanced Green Fluorescent Protein (eGFP) transfected cells in a custom bioreactor. The imaging system has resolved consistently the cellular autofluorescence from that of tissue matrix in situ based on the lifetime fluorescence properties of endogenous molecular species. The location of the re-cellularized area was validated by the eGFP emission. Current results demonstrate the potential of this system as a valuable tool in tissue engineering for in situ studies of cell-tissue interactions in cylindrical or other 3-dimensional structures.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7539617/pdf
Ending Page	044003
Page Count	1
Starting Page	044003
e-ISSN	20506120
DOI	10.1088/2050-6120/ab4342
Journal	Methods and Applications in Fluorescence
Issue Number	4
Volume Number	7
Language	English
Publisher	IOP Publishing
Publisher Date	2019-09-20
Access Restriction	Open
Subject Keyword	Journal: Methods and Applications in Fluorescence Tissue Engineered Vascular Graft Non-destructive Imaging
Content Type	Text
Resource Type	Article

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