Aptamer-displaying peptide amphiphile micelles as a cell-targeted delivery vehicle of peptide cargoes

Content Provider	Scilit
Author	Smith, Josiah D. Cardwell, Leah N. Porciani, David Nguyen, Julie A. Zhang, Rui Gallazzi, Fabio Tata, Rama Rao Burke, Donald H. Daniels, Mark A. Ulery, Bret D.
Copyright Year	2018
Description	Journal: Physical Biology Peptide amphiphile micelles (PAMs) are attractive vehicles for the delivery of a variety of therapeutic and prophylactic peptides. However, a key limitation of PAMs is their lack of preferential targeting ability. In this paper, we describe our design of a PAM system that incorporates a DNA oligonucleotide amphiphile (antitail amphiphile - AA) to form A/PAMs. A cell-targeting DNA aptamer with a 3' extension sequence (tail) complementary to the AA is annealed to the surface to form aptamer-displaying PAMs (Aptamer~A/PAMs). Aptamer~A/PAMs are small, anionic, stable nanoparticles capable of delivering a large mass percentage peptide amphiphile (PA) compared to targeting DNA components. Aptamer~A/PAMs are stable for over 4 hours in the presence of biological fluids. Additionally, the aptamer retains its cell-targeting properties when annealed to the A/PAM, thus leading to enhanced delivery to a specifically-targeted B-cell leukemia cell line. This exciting modular technology can be readily used with a library of different targeting aptamers and PAs, capable of improving the bioavailability and potency of the peptide cargo.
Related Links	http://iopscience.iop.org/article/10.1088/1478-3975/aadb68/pdf
ISSN	14783967
e-ISSN	14783975
DOI	10.1088/1478-3975/aadb68
Journal	Physical Biology
Issue Number	6
Volume Number	15
Language	English
Publisher	IOP Publishing
Publisher Date	2018-08-20
Access Restriction	Open
Subject Keyword	Journal: Physical Biology Peptide Amphiphile Micelle
Content Type	Text
Resource Type	Article
Subject	Cell Biology Structural Biology Molecular Biology Biophysics