NDLI: The effect of cryoprotection on the use of PLGA encapsulated iron oxide nanoparticles for magnetic cell labeling

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The effect of cryoprotection on the use of PLGA encapsulated iron oxide nanoparticles for magnetic cell labeling

Content Provider	Scilit
Author	Tang, Kevin S. Hashmi, Sara M. Shapiro, Erik M.
Copyright Year	2013
Description	Journal: Nanotechnology Magnetic PLGA nanoparticles are a significant advancement in the quest to translate MRI-based cell tracking to the clinic. The benefits of these types of particles are that they encapsulate large amounts of iron oxide nanocrystals within an FDA-approved polymer matrix, combining the best aspects of inert micron-sized iron oxide particles, or MPIOs, and biodegradable small particles of iron oxide, or SPIOs. Practically, PLGA nanoparticle fabrication and storage requires some form of cryoprotectant to both protect the particle during freeze drying and to promote resuspension. While this is a commonly employed procedure in the fabrication of drug loaded PLGA nanoparticles, it has yet to be investigated for magnetic particles and what effect this might have on internalization of magnetic particles. As such, in this study, magnetic PLGA nanoparticles were fabricated with various concentrations of two common cryoprotectants, dextrose and sucrose, and analyzed for their ability to magnetically label cells. It was found that cryoprotection with either sugar significantly enhanced the ability to resuspend nanoparticles without aggregation. Magnetic cell labeling was impacted by sugar concentration, with higher sugar concentrations used during freeze drying more significantly reducing magnetic cell labeling than lower concentrations. These studies suggest that cryoprotection with 1% dextrose is an optimal compromise that preserves monodispersity following resuspension and high magnetic cell labeling.
Related Links	http://europepmc.org/articles/pmc5026304?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5026304/pdf
Ending Page	125101
Page Count	1
Starting Page	125101
ISSN	09574484
e-ISSN	13616528
DOI	10.1088/0957-4484/24/12/125101
Journal	Nanotechnology
Issue Number	12
Volume Number	24
Language	English
Publisher	IOP Publishing
Publisher Date	2013-03-29
Access Restriction	Open
Subject Keyword	Journal: Nanotechnology Magnetically Label Magnetic Cell Labeling
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Mechanics of Materials Mechanical Engineering Bioengineering Electrical and Electronic Engineering

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