NDLI: Single molecule localization imaging of exosomes using blinking silicon quantum dots

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Single molecule localization imaging of exosomes using blinking silicon quantum dots

Content Provider	Scilit
Author	Zong, Shenfei Zong, Junzhu Chen, Chen Jiang, Xiaoyue Zhang, Yizhi Wang, Zhuyuan Cui, Yiping
Copyright Year	2017
Description	Journal: Nanotechnology Discovering new fluorophores which are suitable for single molecule localization microscopy (SMLM) is important for promoting the applications of SMLM in biological or material sciences. Here, we found that silicon quantum dots (Si QDs) possess a fluorescence blinking behavior, making them an excellent candidate for SMLM. The Si QDs are fabricated using a facile microwave-assisted method. Blinking of Si QDs is confirmed by single particle fluorescence measurement and the spatial resolution achieved is about 30 nm. To explore the potential application of Si QDs as the nanoprobes for SMLM imaging, cell derived exosomes are chosen as the object owing to their small size (50-100 nm in diameter). Since CD63 is commonly presented on the membrane of exosomes, CD63 aptamers are attached to the surface of Si QDs to form nanoprobes which can specifically recognize exosomes. SMLM imaging shows that Si QDs based nanoprobes can indeed realize super resolved optical imaging of exosomes. More importantly, blinking of Si QDs is observed in water or PBS buffer with no need for special imaging buffers. Besides, considering that silicon is highly biocompatible, Si QDs should have minimal cytotoxicity. These features make Si QDs quite suitable for SMLM applications especially for live cell imaging.
Related Links	http://iopscience.iop.org/article/10.1088/1361-6528/aaa375/pdf
ISSN	09574484
e-ISSN	13616528
DOI	10.1088/1361-6528/aaa375
Journal	Nanotechnology
Issue Number	6
Volume Number	29
Language	English
Publisher	IOP Publishing
Publisher Date	2017-12-21
Access Restriction	Open
Subject Keyword	Journal: Nanotechnology Silicon Quantum Dots Single Molecule Localization Imaging
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Mechanics of Materials Mechanical Engineering Bioengineering Electrical and Electronic Engineering

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