NDLI: Capillary electrophoresis of adenosine phosphates using boron-doped diamond electrodes

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Capillary electrophoresis of adenosine phosphates using boron-doped diamond electrodes

Content Provider	Scilit
Author	Firmansyah, B. D. Ivandini, Tribidasari A. Gunlazuardi, Jarnuzi
Copyright Year	2017
Description	Journal: Iop Conference Series: Materials Science and Engineering A capillary electrophoresis coupled with electrochemical detection using boron-doped diamond electrode was developed for simultaneous detection of adenosine phosphates, i.e. adenosine monophosphate (AMP), adenosine diphosphate (ADP), and adenosine triphosphate (ATP). In phosphate buffer solution pH 7, these three adenosine phosphates have similar oxidation potentials at around +0.9 V (vs. Ag/AgCl), which indicated that the oxidation occurred at the same moiety. Capillary electrophoresis, which was then performed using fused silica capillary (dia. 0.05 mm) at an applied potential of 10 KV can separate ATP, ADP and AMP with the retention times of 848 s, 1202 s, and 1439 s, respectively. Linear calibration curves with the limits of detection of 0.59 μM, 0.56 μM and 1.78 μM, respectively, can be achieved, suggested that capillary electrophoresis with electrochemical detector is promising for simultaneous detection of adenosine phosphates.
Related Links	http://iopscience.iop.org/article/10.1088/1757-899X/188/1/012052/pdf
ISSN	17578981
e-ISSN	1757899X
DOI	10.1088/1757-899x/188/1/012052
Journal	Iop Conference Series: Materials Science and Engineering
Issue Number	1
Volume Number	188
Language	English
Publisher	IOP Publishing
Publisher Date	2017-04-01
Access Restriction	Open
Subject Keyword	Journal: Iop Conference Series: Materials Science and Engineering Analytical Chemistry Capillary Electrophoresis Adenosine Phosphates Using Boron Doped Diamond Diamond Electrode Boron Doped
Content Type	Text
Resource Type	Article

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