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Measurement of exhaled volatile organic compounds from patients with chronic obstructive pulmonary disease (COPD) using closed gas loop GC-IMS and GC-APCI-MS
| Content Provider | Scilit |
|---|---|
| Author | Allers, M. Langejuergen, Jens Gaida, A. Holz, O. Schuchardt, S. Hohlfeld, Jens Zimmermann, Stefan |
| Copyright Year | 2016 |
| Description | Journal: Journal of Breath Research Due to its high sensitivity, compact size and low cost ion mobility spectrometry (IMS) has the potential to become a point-of-care breath analyzer. Therefore, we developed a prototype of a compact, closed gas loop IMS with gas chromatographic (GC) pre-separation and high resolving power of R = 90. In this study, we evaluated the performance of this GC-IMS under clinical conditions in a COPD study to find correlations between VOCs (10 $ppb_{v}$ to 1 $ppm_{v}$) and COPD. Furthermore, in order to investigate possible correlations between ultra-low concentrated breath VOCs (0.1 $ppt_{v}$ to 1 $ppb_{v}$) and COPD, a modified mass spectrometer (MS) with atmospheric pressure chemical ionization (APCI) and GC pre-separation (GC-APCI-MS) was used. The GC-IMS has been used in 58 subjects (21 smokers with moderate COPD, 12 ex-smokers with COPD, 16 healthy smokers and 9 non-smokers). GC-APCI-MS data were available for 94 subjects (21 smokers with moderate COPD, 25 ex-smokers with COPD, 25 healthy smokers and 23 non-smokers). For 44 subjects, a comparison between GC-IMS and GC-APCI-MS data could be performed. Due to service intervals, subject availability and corrupt data, patient numbers were different for GC-APCI-MS and GC-IMS measurements. Using GC-IMS, three VOCs have been found showing a significant difference between healthy controls and patients with COPD. In the GC-APCI-MS data, we only observed one distinctive VOC, which has been identified as 2-pentanone. This proof-of-principle study shows the potential of our high-resolution GC-IMS in the clinical environment. Due to different linear dynamic response ranges, the data of GC-IMS and GC-APCI-MS were only comparable to a limited extent. |
| Related Links | http://iopscience.iop.org/article/10.1088/1752-7155/10/2/026004/pdf |
| Ending Page | 026004 |
| Page Count | 1 |
| Starting Page | 026004 |
| ISSN | 17527155 |
| e-ISSN | 17527163 |
| DOI | 10.1088/1752-7155/10/2/026004 |
| Journal | Journal of Breath Research |
| Issue Number | 2 |
| Volume Number | 10 |
| Language | English |
| Publisher | IOP Publishing |
| Publisher Date | 2016-04-08 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Breath Research Analytical Chemistry Gc Ims Gc Apci Ms |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pulmonary and Respiratory Medicine |
