NDLI: Photoluminescent functionalized carbon dots for CRISPR delivery: synthesis, optimization and cellular investigation

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Photoluminescent functionalized carbon dots for CRISPR delivery: synthesis, optimization and cellular investigation

Content Provider	Scilit
Author	Hasanzadeh, Akbar Radmanesh, Fatemeh Kiani, Jafar Bayandori, Mehrdad Fatahi, Yousef Aref, Amir R. Karimi, Mahdi
Copyright Year	2019
Description	Journal: Nanotechnology Gene therapy with using of plasmid CRISPR (pCRISPR) reduces mistakes in gene editing and prevents of engendering integrational mutagenesis that have seen through available genome engineering technologies. Developing an ideal and traceable nanocarrier which can accurately and efficiently transfer this complex into the cytosol and facilitates the journey towards the nucleus is a fascinating area of research. Polyethylenimine (PEI) functionalized carbon dots (CD-PEI) were fabricated by one-step microwave assisted pyrolysis with average size around 3 nm. This CD-PEI was shown a good potential for intracellular delivery of genetic materials (~70%). Also, this CD-PEI with passive surface modification with low molecular PEI (2 KDa) has a very high quantum yield as high as 40 percent with low cytotoxicity. The expression rate of the pCRISPR was around 15% in the HEK-293 cell which is comparable with the pristine PEI. Furthermore, the CD-PEI showed the good properties such as high quantum yield, biocompatibility and tunable emission wavelengths, suggesting the potential application of photoluminescent functionalized carbon dots as a suitable traceable nanocarrier for CRISPR delivery.
Related Links	http://iopscience.iop.org/article/10.1088/1361-6528/aafbf9/pdf
ISSN	09574484
e-ISSN	13616528
DOI	10.1088/1361-6528/aafbf9
Journal	Nanotechnology
Issue Number	13
Volume Number	30
Language	English
Publisher	IOP Publishing
Publisher Date	2019-01-04
Access Restriction	Open
Subject Keyword	Journal: Nanotechnology Polyethylenimine (pei)
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Mechanics of Materials Mechanical Engineering Bioengineering Electrical and Electronic Engineering

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