NDLI: Distinct Regulation of Myoblast Differentiation by Intracellular and Extracellular Fibroblast Growth Factor-1

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Distinct Regulation of Myoblast Differentiation by Intracellular and Extracellular Fibroblast Growth Factor-1

Content Provider	Scilit
Author	Uruno, Takehito Oki, Junko Ozawa, Kazuo Miyakawa, Kazuko Ueno, Hikaru Imamura, Toru
Copyright Year	1999
Description	Journal: Growth Factors We studied the role of fibroblast growth factor (FGF)-1 in the physiology of myoblast differentiation. We found that, while endogenous FGF-1 in L6-10 rat myoblasts did not suppress the progress of differentiation, the addition of FGF-1 to the culture medium suppressed it. Moreover, L6-10 cells stably transfected with full length FGF-1 undergo enhanced differentiation. The latter was well correlated with myogenin expression and myotube formation. Constitutive expression of a mutant FGF-1 (FGF-1 U) that lacked a nuclear localization signal, promoted the differentiation of the myoblasts even more strongly. Furthermore, the expression of FGF-1 U in an inducible expression system enhanced myogenin expression promptly. In L6-10 transfectants expressing a dominant-negative mutant of FGF receptor, stable transfection of FGF-1 promoted differentiation as it did in parent cells. Studies with FGF receptors and MAP kinase suggest that both are involved in the effect of FGF-1 when it is supplemented to culture medium but not during the effect of endogenous FGF-1 synthesized in cells. We conclude that intracellular (endogenous) and extracellular (exogenous) FGF-1 have differential effects on the regulation of myogenic differentiation of L6-10 cells.
Related Links	https://www.tandfonline.com/doi/pdf/10.3109/08977199909103519
ISSN	08977194
e-ISSN	10292292
DOI	10.3109/08977199909103519
Journal	Growth Factors
Issue Number	2
Volume Number	17
Language	English
Publisher	Informa UK Limited
Publisher Date	1999-01-01
Access Restriction	Open
Subject Keyword	Journal: Growth Factors Endocrinology and Metabolism Fibroblast Growth Factor (fgf) Differentiation
Content Type	Text
Subject	Cell Biology Clinical Biochemistry Endocrinology

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