Cell Cycle-Regulated Centers of DNA Double-Strand Break Repair

Content Provider	Scilit
Author	Lisby, Michael de Mayolo, Adriana Antúnez Mortensen, Uffe H. Rothstein, Rodney
Copyright Year	2003
Description	Journal: Cell Cycle In eukaryotes, homologous recombination is an important pathway for the repair of DNA double-strand breaks. We have studied this process in living cells in the yeast Saccharomyces cerevisiae using Rad52 as a cell biological marker. In response to DNA damage, Rad52 redistributes itself and forms foci specifically during S phase. We have shown previously that Rad52 foci are centers of DNA repair where multiple DNA double-strand breaks colocalize. Here we report a correlation between the timing of Rad52 focus formation and modification of the Rad52 protein. In addition, we show that the two ends of a double-strand break are held tightly together in the majority of cells. Interestingly, in a small but significant fraction of the S phase cells, the two ends of a break separate suggesting that mechanisms exist to reassociate and align these ends for proper DNA repair.
Related Links	https://www.tandfonline.com/doi/pdf/10.4161/cc.2.5.483?needAccess=true
Ending Page	481
Page Count	5
Starting Page	477
ISSN	15384101
e-ISSN	15514005
DOI	10.4161/cc.2.5.483
Journal	Cell Cycle
Issue Number	5
Volume Number	2
Language	English
Publisher	Informa UK Limited
Publisher Date	2003-09-12
Access Restriction	Open
Subject Keyword	Journal: Cell Cycle Biochemistry and Molecular Biology Double Strand Breaks
Content Type	Text
Subject	Cell Biology Developmental Biology Medicine Molecular Biology