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Suppressive and Enhancing Effect of T-2 Toxin on Murine Lymphocyte Activation and Interleukin 2 Production
| Content Provider | Scilit |
|---|---|
| Author | Holt, P. S. Corrier, D. E. Deloach, J. R. |
| Copyright Year | 1988 |
| Description | Journal: Immunopharmacology and Immunotoxicology T-2 toxin, a fungal metabolite shown previously to exert potent immunosuppressive effects, was examined for its effects on activation and interleukin 2 (IL 2) production by murine and rat splenocytes. Splenocytes (1 X 10(6) cells/well) were incubated with 1 microgram Concanavalin A (Con A) for 48 h at which time cellular protein and DNA synthesis by these cells were ascertained using radiolabeled precursors. IL 2 synthesis was determined from the cell supernatant using the IL 2-requiring cell line CTLL. Spleen cells from mice treated for 4 consecutive days with 2 mg/kg toxin exhibited a 50% reduction in in vitro Con A activation but the supernatant IL 2 levels from these cells was 4-fold higher than cells from control mice. In vitro exposure of Con A-activated normal spleen cells to various toxin doses for 48 h resulted in diminished protein and DNA synthesis at 0.4 ng toxin with maximum inhibition at 1 ng (50% inhibition (TCID50) = 0.5 ng). Enhanced synthesis of both products was observed at lower toxin concentrations. IL 2 production by these cells followed a similar toxin dose response. Rat splenocytes were slightly more resistant and CTLL cells were slightly more sensitive to T-2 toxin than mouse splenocytes. These results indicate the variable effects a cytotoxic agent can have on lymphoid cells and that dosage is an important parameter for these effects. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/08923978809041427 |
| Ending Page | 385 |
| Page Count | 21 |
| Starting Page | 365 |
| ISSN | 08923973 |
| e-ISSN | 15322513 |
| DOI | 10.3109/08923978809041427 |
| Journal | Immunopharmacology and Immunotoxicology |
| Issue Number | 3 |
| Volume Number | 10 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1988-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Immunopharmacology and Immunotoxicology Immunosuppressive Cytotoxic Agent Activation and Interleukin Dna Synthesis Rat Splenocytes |
| Content Type | Text |
| Subject | Immunology and Allergy Toxicology Pharmacology Immunology |
