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Cancer cell spheroids as a model to evaluate chemotherapy protocols
| Content Provider | Scilit |
|---|---|
| Author | Perche, Federico Torchilin, Vladimir P. |
| Copyright Year | 2012 |
| Description | Journal: Cancer Biology & Therapy To determine whether the spheroid culture can be used to evaluate drug efficacy, we have evaluated the toxicity of free or carrier-associated doxorubicin as a single drug or in combination with other antineoplastic agents using the spheroid cultures of drug-resistant cancer cells. Paclitaxel, cisplatin, dexamethasone, mitoxantrone, sclareol or methotrexate were used in combination with doxorubicin. The effect of the treatment protocols on free, micellar and liposomal doxorubicin accumulation in spheroids and on resulting toxicity was evaluated by fluorescence and lactate dehydrogenase release, respectively. Enhanced doxorubicin accumulation and toxicity were observed after spheroid pretreatment with mitoxantrone or paclitaxel. Effects of the drug combination with doxorubicin were sequence dependent, use of doxorubicin as the first drug being the least inducer of toxicity. Finally, spheroids were recognized by a cancer cell-specific antibody. Our results suggest the usefulness of spheroids to evaluate chemotherapy combinations. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469478/pdf |
| Ending Page | 1213 |
| Page Count | 9 |
| Starting Page | 1205 |
| ISSN | 15384047 |
| e-ISSN | 15558576 |
| DOI | 10.4161/cbt.21353 |
| Journal | Cancer Biology & Therapy |
| Issue Number | 12 |
| Volume Number | 13 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2012-10-27 |
| Access Restriction | Open |
| Subject Keyword | Journal: Cancer Biology & Therapy Pharmacology and Pharmacy Cancer Cell Spheroids Mitoxantrone |
| Content Type | Text |
| Subject | Cancer Research Pharmacology Molecular Medicine Oncology |
