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Optimizing acth-stimulated steroid secretion by cultured adrenocortical tumor cells
| Content Provider | Scilit |
|---|---|
| Author | Mathias, S. Wei, L. Hunter, E. Wells, O. Mgbonyebi, O. Mrotek, J. |
| Copyright Year | 1995 |
| Description | Journal: Endocrine research If 1 mM supplemental $Ca^{++}$, or 1.6 mg hemoglobin (Hb)/ml medium (23.5 uM, a concentration comparable to that in 1 ml mouse blood), was added to Eagle's mimimum essential medium (MEM) (which contains 1 mM $Ca^{++}$), basal and maximally stimulated 20-dihydroprogesterone (20-DHP) secretion by cultured Y-1 mouse adrenal tumor cells [49 - 65th passages] could be measured by radioimmunoassay after a 0.5 hr incubation. ACTH-stimulated, but not basal, 20-DHP secretion increased after 1 mM $Ca^{++}$ treatment. 5 mM EGTA significantly reduced basal and stimulated 20-DHP secretion, although significant ACTH stimulation still remained. Basal and stimulated secretion significantly increased when Hb was present. Although $O_{2}$ involvement in the Hb effect was tested by bubbling medium with 100% $O_{2}$ for 10 minutes, basal and stimulated secretion was unaffected. Since proteins, such as Hb, non-specifically bind free steroids, enhancing secretion, albumin (A1) was compared to Hb. A1 enhanced unstimulated, but not ACTH-stimulated, 20-DHP secretion. The Hb effect may not be due to non-specific protein-steroid binding. $Ca^{++}$ supplementation and chelation studies suggest the necessity to optimize co-factors in steroidogenic tissue incubation media to maximize basal and stimulated steroid synthesis and secretion. Since physiologically relevant Hb levels enhanced basal and stimulated Y-1 cell steroid secretion by mechanisms other than protein-steroid binding and soluble $O_{2}$ had little effect, Hb may more efficiently transport $O_{2}$ to cultured cells than soluble $O_{2}$ diffusion through medium does. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.3109/07435809509030428 |
| Ending Page | 127 |
| Page Count | 7 |
| Starting Page | 121 |
| ISSN | 07435800 |
| e-ISSN | 15324206 |
| DOI | 10.3109/07435809509030428 |
| Journal | Endocrine research |
| Issue Number | 1-2 |
| Volume Number | 21 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1995-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Endocrine research Cultured Cells Supplemental |
| Content Type | Text |
| Subject | Medicine Endocrinology |
