NDLI: Expression - Level Dependent Activation of Recombinant Human Parathyroid Hormone/Parathyroid Hormone-Related Peptide Receptor: Effect of Human Parathyroid Hormone (1-34), (1-31), and (28-48)

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Expression - Level Dependent Activation of Recombinant Human Parathyroid Hormone/Parathyroid Hormone-Related Peptide Receptor: Effect of Human Parathyroid Hormone (1-34), (1-31), and (28-48)

Content Provider	Scilit
Author	Tonn, Oliver Rriegbaum, Sepp Braitmaier, Andrea Schäfer, Wolfgang Esswein, Angelika Dony, Carola Kaluza, Klaus Honold, Konrad
Copyright Year	2000
Description	Journal: Journal of Receptors and Signal Transduction A stable recombinant chinese hamster ovary (CHO) cell model system expressing the human type-1 receptor for parathyroid hormone and parathyroid hormone-related peptide (hPTH-R) was established for the analysis of human PTH (hPTH) variants. The cell lines showed receptor expression in the range from 10(5) to I.9 x 10(6) receptors per cell. The affinity of the receptors for hPTH-(1-34) was independent of the receptor number per cell (Kd approximately = 8 nmol/1). The induction of cAMP by hPTH-(1-34) is maximal in clones expressing >2x10(5) receptors per cell and Ca++ signals were maximal in cell lines expressing >1.4x10(6) receptors per cell. Second messenger specific inhibitors demonstrated that PTH-induced increases in intracellular cAMP and Ca++ are independent and Ca++ ions are derived from intracellular stores. The cAMP-specific receptor activator hPTH-(1-31) showed also an increase in intracellular Ca++. Even in cell lines expressing more than 10(6) receptors per cell the Ca++/PKC specific activator hPTH-(28-48) did not activate hPTH-Rs. Based on these results, synthesis of further derivatives of PTH is required to identify pathway-specific ligands for the type-1 hPTH-R.
Related Links	http://www.tandfonline.com/doi/pdf/10.3109/10799890009150641
Ending Page	151
Page Count	19
Starting Page	133
ISSN	10799893
e-ISSN	15324281
DOI	10.3109/10799890009150641
Journal	Journal of Receptors and Signal Transduction
Issue Number	2-3
Volume Number	20
Language	English
Publisher	Informa UK Limited
Publisher Date	2000-01-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Receptors and Signal Transduction Intracellular Parathyroid Hormone
Content Type	Text
Subject	Cell Biology Biochemistry Molecular Biology

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Expression - Level Dependent Activation of Recombinant Human Parathyroid Hormone/Parathyroid Hormone-Related Peptide Receptor: Effect of Human Parathyroid Hormone (1-34), (1-31), and (28-48)

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