Detection of Olfactory Receptor I7 Self‐Assembled Multilayer Formation and Immobilization Using a Quartz Crystal Microbalance

Content Provider	Scilit
Author	Rodriguez-Segui, Santiago A. Pla, M. Minic, J. Pajot‐Augy, E. Salesse, R. Hou, Yanxia Jaffrezic‐Renault, N. Mills, C. A. Samitier, J. Errachid, A.
Copyright Year	2006
Description	Journal: Analytical Letters A self‐assembled multilayer based on a mixed MHDA‐Biotinyl PE self‐assembled monolayer followed by the addition of a biotin‐avidin system was built up on the gold electrode of a quartz crystal microbalance, which was used to monitor the deposition. With the view to producing an odorant sensing device, an olfactory receptor (OR), I7 OR, was immobilized on the self‐assembled multilayer. The I7 OR originates from a large group of proteins belonging to the I subfamily of G protein–coupled receptors that binds odorant ligands. All formation steps were followed with the quartz crystal microbalance with dissipation measurements (QCM‐D). Valuable information was obtained regarding the composition of each layer, providing evidence of the high dissipation effect of nanosomes adhesion. Also, based on the results, an explanation for multilayer formation and binding relations between components is proposed.
Related Links	https://core.ac.uk/download/pdf/18322816.pdf
Ending Page	1745
Page Count	11
Starting Page	1735
ISSN	00032719
e-ISSN	1532236X
DOI	10.1080/00032710600714030
Journal	Analytical Letters
Issue Number	8
Volume Number	39
Language	English
Publisher	Informa UK Limited
Publisher Date	2006-07-01
Access Restriction	Open
Subject Keyword	Journal: Analytical Letters Olfactory Receptor Quartz Crystal Microbalance (qcm) Self‐assembled Multilayer
Content Type	Text
Subject	Biochemistry (medical) Spectroscopy Analytical Chemistry Clinical Biochemistry Biochemistry Electrochemistry