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Long Non-Coding RNA AGAP2-AS1/miR-628-5p/PTN Axis Modulates Proliferation, Migration, Invasion, and Apoptosis of Glioma Cells
| Content Provider | Scilit |
|---|---|
| Author | Yan, Yang Wang, Yiping Liu, Yuxia Chen, Tao Zhu, Yaoli Li, Huiqing Kong, Fangen |
| Copyright Year | 2020 |
| Description | Journal: Cancer Management and Research Purpose: Long non-coding RNAs (lncRNAs) have been reported to be involved in a variety of cancers, including glioma. However, the exact role and underlying mechanism of lncRNA AGAP2 antisense RNA 1 (AGAP2-AS1) in glioma have not yet been fully elucidated. Methods: The expression levels of AGAP2-AS1, microRNA-628-5p (miR-628-5p) and pleiotrophin (PTN) were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation, apoptosis, migration and invasion were detected by Cell Counting Kit-8 (CCK-8) assay, flow cytometry, transwell assay, respectively. Western blot assay was used to detect the protein level of PTN. The interaction between miR-628-5p and AGAP2-AS1 or PTN was predicted by bioinformatics software and confirmed by the dual-luciferase reporter and RNA Immunoprecipitation (RIP) assays. Murine xenograft model was established to confirm the role of AGAP2-AS1 in glioma progression in vivo. Results: AGAP2-AS1 expression was upregulated in glioma tissues and cells. Knockdown of AGAP2-AS1 inhibited the proliferation, migration and invasion, but facilitated apoptosis in glioma cells. Moreover, AGAP2-AS1 could directly bind to miR-628-5p and its overexpression reversed the anti-tumor effect of miR-628-5p restoration on the progression of glioma cells. In addition, miR-628-5p directly targeted PTN and its inhibition abolished the inhibitory effect of PTN knockdown on the progression of glioma cells. Furthermore, AGAP2-AS1 functioned as a competing endogenous RNA (ceRNA) by sponging miR-628-5p to modulate PTN expression. Besides, AGAP2-AS1 depletion reduced tumor growth by upregulating miR-628-5p and downregulating PTN. Conclusion: AGAP2-AS1 knockdown suppressed cell proliferation, migration and invasion but promoted cell apoptosis in glioma cells by regulating miR-628-5p/PTN axis, providing novel avenues for treatment of glioma. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7398883/pdf https://www.dovepress.com/getfile.php?fileID=59823 |
| Ending Page | 6068 |
| Page Count | 10 |
| Starting Page | 6059 |
| ISSN | 11791322 |
| DOI | 10.2147/cmar.s250890 |
| Journal | Cancer Management and Research |
| Volume Number | ume 12 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-07-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Cancer Management and Research Cell Progression |
| Content Type | Text |
| Subject | Oncology |
