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Further Characterization of Glutaminase Isozymes fromPseudomonas aeruginosa
| Content Provider | Scilit |
|---|---|
| Author | Ohshima, Matsumi Yamamoto, Tatsuo Soda, Kenji |
| Copyright Year | 1976 |
| Description | Journal: Bioscience, Biotechnology, and Biochemistry (1) Both glutaminases A and B of Pseudomonas aeruginosa are inactivated by urea and guanidine hydrochloride, and the activities are partially restored by removal of the denaturants, while sodium lauryl sulfate denatured irreversibly the isozymes. (2) Glutaminase A consists of 4 identical subunits (mol. wt, 35,000) and B is composed of one polypeptide chain (mol. wt., 67,000). (3) Glutaminase A, which catalyzes the hydrolysis and also the hydroxylaminolysis of L and D isomers of glutamine and asparagine, does not act on γ-N-substituted glutamine e.g., γ-glutamylhydrazide. Some l- and d-γ-glutamyl derivatives, e.g., l- and d-γ-glutamyl-hydrazide, l- and d-γ-glutamylmethylester, and l-γ-glutamyl-l-alanine are substrates for glutaminase B, which does not catalyze the hydrolysis and hydroxylaminolysis of asparagine. α-Amino adipamic acid and α-amino substituted amino acids are inert for both the isozymes. (4) The acylation step is rate-limiting in the catalytic reactions by both the isozymes. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/00021369.1976.10862383?needAccess=true |
| Ending Page | 2256 |
| Page Count | 6 |
| Starting Page | 2251 |
| ISSN | 09168451 |
| e-ISSN | 13476947 |
| DOI | 10.1080/00021369.1976.10862383 |
| Journal | Bioscience, Biotechnology, and Biochemistry |
| Issue Number | 11 |
| Volume Number | 40 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1976-11-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Bioscience, Biotechnology, and Biochemistry Biochemistry and Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Molecular Biology Biochemistry Applied Microbiology and Biotechnology Biotechnology |
