Loading...
Please wait, while we are loading the content...
Similar Documents
Characterization of enzyme immobilization in liposomes prepared from proliposomes
| Content Provider | Scilit |
|---|---|
| Author | Dufour, P. Vuillemard, J. C. Laloy, E. Simard, R. E. |
| Copyright Year | 1996 |
| Description | Journal: Journal of Microencapsulation This study investigated the influence of ionic strength, liposome net charge and enzyme concentration on the immobilization of chymotrypsin in liposomes obtained from proliposomes. Depending on ionic strength and chymotrypsin concentration, immobilization efficiencies (IE) as high as 96 and 68% were obtained for liposomes prepared with Pro-lipo 3045 S and Pro-lipo 3080 S respectively. Increasing ionic strength and enzyme concentration resulted in a decrease in IE for both types of liposomes, and this was more pronounced for ionic strength. Relatively high amounts of chymotrypsin were found to be immobilized on the surface of the liposomes. Hydrophobic interactions between chymotrypsin and the hydrophobic tails of the phospholipids during liposome formation were probably responsible for this phenomenon. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/02652049609052906 |
| Ending Page | 194 |
| Page Count | 10 |
| Starting Page | 185 |
| ISSN | 02652048 |
| e-ISSN | 14645246 |
| DOI | 10.3109/02652049609052906 |
| Journal | Journal of Microencapsulation |
| Issue Number | 2 |
| Volume Number | 13 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1996-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Microencapsulation Food Science and Technology Enzyme Immobilization Chymotrypsin |
| Content Type | Text |
| Subject | Organic Chemistry Colloid and Surface Chemistry Physical and Theoretical Chemistry Bioengineering Pharmaceutical Science |
