NDLI: Effects of 50 Hz electromagnetic fields on human epidermal stem cells cultured on collagen sponge scaffolds

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Effects of 50 Hz electromagnetic fields on human epidermal stem cells cultured on collagen sponge scaffolds

Content Provider	Scilit
Author	Bai, Wen-Fang Zhang, Ming-Sheng Huang, Hong Zhu, Hong-Xiang Xu, Wei-Cheng
Copyright Year	2012
Description	Journal: International Journal of Radiation Biology Purpose: This study is to investigate the effects of electromagnetic fields (EMF) on proliferation of epidermal stem cells (ESC), which could present a viable clinical option for skin tissue engineering. Materials and methods: The ESC obtained from human foreskin were grafted into type-I three-dimensional collagen sponge scaffolds, and then were exposed with EMF (frequency 50 Hz, intensity 5 mT) for 14 d (30 min per d). Meanwhile, the control group was set under the same conditions without EMF. The effects of EMF on growth and proliferation of ESC were analyzed with staining of hematoxylin and eosin (H\u26E) and 4′,6-diamidino-2-phenylindole (DAPI) under microscope or scanning electron microscope. The data of DAPI staining for 2 d, 7 d, 10 d and 14 d were collected respectively to investigate the cells proliferation. Results: ESC cultured in collagen sponge scaffolds could be steady grown and EMF could promote ESC proliferation compared with control (P \u3c 0.05). Conclusions: EMF could significantly promote proliferation of ESC, which leads to a promising clinical option for skin tissue engineering
Related Links	http://www.tandfonline.com/doi/pdf/10.3109/09553002.2012.692496
Ending Page	530
Page Count	8
Starting Page	523
ISSN	09553002
e-ISSN	13623095
DOI	10.3109/09553002.2012.692496
Journal	International Journal of Radiation Biology
Issue Number	7
Volume Number	88
Language	English
Publisher	Informa UK Limited
Publisher Date	2012-04-30
Access Restriction	Open
Subject Keyword	Journal: International Journal of Radiation Biology Rehabilitation Cells Cultured Collagen Sponge Sponge Scaffolds Proliferation Cultured in Collagen
Content Type	Text
Subject	Radiology, Nuclear Medicine and Imaging Radiological and Ultrasound Technology

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