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Improved Sensitivity for High Resolution in Situ Hybridization Using Resin Extraction of Methyl Methacrylate Embedded Material
| Content Provider | Scilit |
|---|---|
| Author | Saito, Chieko Ashi, Makoto Hay Sakai, Atsushi Fujie, Makoto Kuroiwa, Haruko Kuroiwa, Tsuneyoshi |
| Copyright Year | 1999 |
| Description | Journal: Biotechnic & Histochemistry An in situ hybridization procedure resulting in both high resolution and sensitivity was established by using the removable methyl methacrylate resin, Technovit 9100. Young bicel-lular pollen of tobacco (Nicotiana tabacum L. SR-1) was embedded in Technovit 9100 resin and sectioned. The resin was extracted with (2-methoxyethyl)-acetate followed by in situ hybridization with cRNA probes to detect cytoplasmic 18S/25S rRNA. Signal intensity obtained by this procedure was approximately twice as great as that obtained by an earlier procedure using Technovit 7100, a glycol methacrylate resin that cannot be removed from sections. This improvement in sensitivity made it possible to observe subcellular localization of small amounts of RNA as revealed by visualization of plastid 23S rRNA in a generative cell of Plumbago auriculata pollen. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.3109/10520299909066476 |
| Ending Page | 48 |
| Page Count | 9 |
| Starting Page | 40 |
| ISSN | 10520295 |
| e-ISSN | 14737760 |
| DOI | 10.3109/10520299909066476 |
| Journal | Biotechnic & Histochemistry |
| Issue Number | 1 |
| Volume Number | 74 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1999-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Biotechnic & Histochemistry Plant Sciences Generative Cell in Situ Hybridization Nicotiana Tabacum Plumbago Auriculata Resin Extraction Technovit 9100 Vegetative Cell |
| Content Type | Text |
| Subject | Histology Medical Laboratory Technology |
