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Loss of miR-204-5p Promotes Tumor Proliferation, Migration, and Invasion Through Targeting YWHAZ/PI3K/AKT Pathway in Esophageal Squamous Cell Carcinoma
| Content Provider | Scilit |
|---|---|
| Author | Shen, Zhimin Chai, Tianci Luo, Fei Liu, Zhun Xu, Hui Zhang, Peipei Kang, Mingqiang Chen, Sui |
| Copyright Year | 2020 |
| Description | Journal: OncoTargets and therapy |
| Abstract | Purpose: MicroRNAs dysregulation has been confirmed in multiple malignancies. This paper reported the molecular mechanism of miR-204-5p in esophageal squamous cell carcinoma (ESCC). Methods: miR-204-5p expression in 30 ESCC tumor tissues and 10 normal tissues was downloaded from RNA-seq data. ESCC tissues/normal tissues of 97 ESCC patients were collected. TE-1 and KYSE510 cells were transfected by miR-204-5p mimic, inhibitor, siYWHAZ or their corresponding controls. The phenotype of cells was detected by CCK-8 assay, transwell experiment, and flow cytometry. Luciferase reporter gene assay and RNA-binding protein immunoprecipitation (RIP) were performed to verify the targeting relationship between miR-204-5p and YWHAZ. miR-204-5p and YWHAZ expression in tissues/cells was detected by qRT-PCR and Western blot. Xenograft tumor experiment was performed. Results: miR-204-5p expression was declined in ESCC patients and cells, which was indicated the poor outcome of patients. Compared with siNC group, TE-1 cells in miR-204-5p inhibitor group had higher OD450 value, less cell percentage in G1 phase, and more cell percentage in S phase, lower apoptosis percentage, and higher migration and invasion cell numbers. Moreover, KYSE510 cells of miR-204-5p mimic group showed lower OD450 value, more cell percentage in G1 phase and less cell percentage in S phase, higher apoptosis percentage, and lower migration and invasion cell numbers than control. YWHAZ was directly inhibited by miR-204-5p. Relative to siNC group, TE-1 cells of miR-inhibitor group exhibited higher YWHAZ protein expression, higher OD450 value, less cell percentage in G1 phase and more cell percentage in S phase, lower apoptosis percentage, higher migration and invasion cell numbers, and higher p-PI3K/PI3K and p-AKT/AKT protein expression, while siYWHAZ rescued the effects of miR-inhibitor. miR-204-5p up-regulation inhibited ESCC growth in vivo. Conclusion: miR-204-5p inhibits ESCC progression by targeted inhibition of YWHAZ/PI3K/AKT. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263804/pdf https://www.dovepress.com/getfile.php?fileID=58435 |
| Ending Page | 4690 |
| Page Count | 12 |
| Starting Page | 4679 |
| ISSN | 11786930 |
| DOI | 10.2147/ott.s243215 |
| Journal | OncoTargets and therapy |
| Volume Number | ume 13 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-05-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: OncoTargets and therapy |
| Content Type | Text |
| Subject | Pharmacology (medical) Oncology |
