Loading...
Please wait, while we are loading the content...
Similar Documents
Ion accumulation and expression of ion homeostasis-related genes associated with halophilism, NaCl-promoted growth in a halophyte Mesembryanthemum crystallinum L.
| Content Provider | Scilit |
|---|---|
| Author | Tran, Dan Q. Konishi, Ayako Cushman, John C. Morokuma, Masahiro Toyota, Masanori Agarie, Sakae |
| Copyright Year | 2019 |
| Description | Journal: Plant Production Science |
| Abstract | A halophyte, the common ice plant (Mesembryanthemum crystallinum L.), shows the maximal growth under salinity, in which almost all crops die. The NaCl-stimulated growth, which is referred to as halophilism, is an important trait for adaptation to salinity, but the mechanism is still unclear. To elucidate factors contributing to the halophilism, we tested the effects of NaCl on growth, ion accumulation, and expression of ion homeostasis-related genes in suspension-cultured cells. Among nine ions analyzed, $Na^{+}$, $Cl^{−}$, $K^{+}$, and NO_{3}$^{−}$ were accumulated significantly in the cells showing halophilism than that in the salt-untreated cells, and the accumulation of these ions was positively correlated with the growth. Also, the expression of ion homeostasis-related genes for plasma membrane transporters and channels for incorporation of nitrate (McNRT1), sodium (McHKT1), potassium (McKmt1), $cations/Cl^{−}$ (McCCC1), for tonoplast antiporters $H^{+}/Cl^{−}$ (McCLC1) and $Na^{+}/H^{+}$ (McNHX1), and V-ATPase subunit c (McVmac1) for sequestration of $Cl^{−}$ and $Na^{+}$ into the vacuole, and for enzymes catalyzing biosynthesis of proline (McP5CS) and ononitol (McImt1) was higher in the cells showing halophilism than that in the salt-untreated cells. These results indicate that the ion accumulation and the expression of ion homeostasis-related genes contribute to the NaCl-stimulated growth enhancement in the halophyte, the common ice plant.Abbreviations: CCC: cation/Cl- cotransporter; CLC: H+/Cl–antiporter; DW: dry weight; FW: fresh weight; HAK: high affinity potassium; HKT: high potassium transporter; Imt: myo-inositol O-methyl transferase; MIP: major intrinsic protein; NHX: Sodium/proton antiporter; P5CS: delta 1-pyrroline-5-carboxylate synthase; PCV: Packed cell volume; PEG: polyethylene glycol; XTH: xyloglucan endotransglucosylase/hydrolase Graphical abstract The growth enhancement of the ice plants by NaCl was positively correlated with $K^{+}$ accumulation and the increased expression of ion homeostasis related genes contribute to the halophilism. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/1343943X.2019.1647788?needAccess=true |
| ISSN | 1343943X |
| e-ISSN | 13491008 |
| DOI | 10.1080/1343943x.2019.1647788 |
| Journal | Plant Production Science |
| Issue Number | 1 |
| Volume Number | 23 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2019-08-09 |
| Access Restriction | Open |
| Subject Keyword | Journal: Plant Production Science Particles and Fields Physics Ion Transporter Mesembryanthemum Crystallinum L |
| Content Type | Text |
| Subject | Agronomy and Crop Science |
