New Fuchsin-Hematoxylin-Eosin; A Stain for Acid-Fast Bacilli and Surrounding Tissue

Content Provider	Scilit
Author	Willigan, Donald A. Garric, V. Anita Trosko, Beverly K.
Copyright Year	1961
Description	Journal: Stain technology This is a staining technique for histopathologic evaluation of tissue reaction in the environs of acid-fast tubercle bacilli (avian and bovine) in sections. Fresh tissue is fixed in 10% neutral formalin and processed in the usual manner for embedding in paraffin. Sections are cut approximately 6 μ. thick, dewaxed, hydrated, and stained with Harris' hematoxylin. They are rinsed in tap water, differentiated in add alcohol, washed in tap water, given a distilled water rinse and stained at 20-30° C in a 1% solution of new fuchsin in 5% phenol. Each slide is then handled individually by placing it directly into a saturated aqueous solution of Li2CO3 and agitated gently for a few seconds. This is followed by differentiation with 5% glacial acetic acid in absolute or 95% ethyl alcohol until the color stops running. Two rinses in absolute or 95% ethyl alcohol follow. The sections are then counterstained in the color add of eosin Y prepared according to the method of Schleicher (Stain Techn., 28, 119-23, 1953) and used as an 0.025% solution in absolute alcohol. Following passage through 2 changes of absolute alcohol, the sections are cleared in xylene, then mounted in Permount or similar synthetic resin. The add-fast barilli are emphasized by their bright retractile red color within a contrasting background of hematoxylin and eosin.
Related Links	http://www.tandfonline.com/doi/pdf/10.3109/10520296109113299
Ending Page	321
Page Count	3
Starting Page	319
ISSN	00389153
DOI	10.3109/10520296109113299
Journal	Stain technology
Issue Number	5
Volume Number	36
Language	English
Publisher	Informa UK Limited
Publisher Date	1961-01-01
Access Restriction	Open
Subject Keyword	Journal: Stain technology Mycobacterium Tuberculosis Rosaniline Dyes Stains AND Staining
Content Type	Text
Subject	Anatomy