Green synthesis of multifunctional PEG-carboxylate π back-bonded gold nanoconjugates for breast cancer treatment

Content Provider	Scilit
Author	Gajendiran, Mani Jo, Heejung Kim, Kyobum Balasubramanian, Sengottuvelan
Copyright Year	2019
Description	Journal: International Journal of Nanomedicine Background: Surface functionalization of gold nanoparticles (AuNPs) has emerged as a promising field of research with enormous biomedical applications. The folate (FA)-attached polymer-gold nanoconjugates play vital role in targeting the cancer cells. Methods: AuNPs were synthesized by using di- or tri-carboxylate-polyethylene glycol (PEG) polymers, including citrate-PEG (CPEG), malate-PEG (MAP), and tartrate-PEG (TAP), as a reducing and stabilizing agent. After synthesis of polymer-AuNPs, the freely available hydroxyl and carboxylate groups of CPEG, MAP, and TAP were used to attach a cancer cell-targeting agent, FA, via a 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxy succinimide coupling reaction to obtain FA-CPEG-AuNP, FA-MAP-AuNP, and FA-TAP-AuNP nanoconjugates, respectively. The 5-fluorouracil (5FU) was attached to π back-bonded carbonyl oxygens of the nanoconjugates, and the in vitro drug release profile was studied by high pressure liquid chromatography. Biocompatibility profiles of the FA-CPEG-AuNP, FA-MAP-AuNP, and FA-TAP-AuNP nanoconjugates were investigated using adult human dermal fibroblasts. Anti-breast cancer activity of 5FU-loaded nanoconjugates was investigated using MCF-7 breast cancer cells. Results: X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy analyses confirmed that AuNPs attached to CPEG, MAP, or TAP via the formation of π back bonding between AuNPs and the ester carbonyl group. The π back-bonded nanoconjugates exhibited sustained release of 5FU up to 27 days. FA-MAP-AuNPs exhibited an $IC_{50}$ at 5 µg/mL, while FA-CPEG-AuNPs and FA-TAP-AuNPs showed the $IC_{50}$ at 100 µg/mL toward MCF-7 cancer cells. Conclusion: The developed polymer π back-bonded multifunctional gold nanoconjugates could be used as a potential drug delivery system for targeting MCF-7 cancer cells.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354699/pdf https://www.dovepress.com/getfile.php?fileID=47748
Ending Page	834
Page Count	16
Starting Page	819
e-ISSN	11782013
DOI	10.2147/ijn.s190946
Journal	International Journal of Nanomedicine
Volume Number	ume 14
Language	English
Publisher	Informa UK Limited
Publisher Date	2019-01-01
Access Restriction	Open
Subject Keyword	Journal: International Journal of Nanomedicine Biomaterials 5-fluorouracil Anticancer Activity Green Synthesis Polymer-gold Nanoconjugates
Content Type	Text