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A Magnesium-Incorporated Nanoporous Titanium Coating for Rapid Osseointegration
| Content Provider | Scilit |
|---|---|
| Author | Li, Xiaodong Wang, Mingyi Zhang, Wenjie Bai, Yuting Liu, Yuan Meng, Jian Zhang, Ling |
| Copyright Year | 2020 |
| Description | Journal: International Journal of Nanomedicine |
| Abstract | Purpose: Micro-arc oxidation (MAO) is a fast and effective method to prepare nanoporous coatings with high biological activity and bonding strength. Simple micro/nano-coatings cannot fully meet the requirements of osteogenesis. To further improve the biological activity of a titanium surface, we successfully added biological magnesium $(Mg^{2+}$) to a coating by micro-arc oxidation and evaluated the optimal magnesium concentration in the electrolyte, biocompatibility, cell adhesion, proliferation, and osteogenesis in vitro. Methods: Nanoporous titanium coatings with different concentrations of magnesium were prepared by micro-arc oxidation and characterized by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). The $Mg^{2+}$ release ability of the magnesium-incorporated nanoporous titanium coatings was determined by inductively coupled plasma emission spectrometry (ICP-OES). The cytotoxicity of the magnesium-incorporated nanoporous titanium coatings was detected with live/dead double-staining tests. A CCK-8 assay was employed to evaluate cell proliferation, and FITC-phalloidin was used to determine the structure of the cytoskeleton by staining β-actin. Alkaline phosphatase (ALP) activity was evaluated by alizarin red S (ARS) staining to determine the effect of the coatings on osteogenic differentiation in vitro. The mRNA expression of osteogenic differentiation-related markers was measured using qRT-PCR. Results: EDS analyses revealed the successful addition of magnesium to the microporous coatings. The best magnesium concentration of the electrolyte for preparing the new coating was determined. The results showed that the nano-coatings prepared using the electrolyte with 2 g/L magnesium acetate best promoted the adhesion, proliferation, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Conclusion: These results suggest that the new titanium metal coating with a dual effect of promoting bone morphology and supplying the biological ion $Mg^{2+}$ can be beneficial for rapid osseointegration. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490434/pdf https://www.dovepress.com/getfile.php?fileID=61262 |
| Ending Page | 6603 |
| Page Count | 11 |
| Starting Page | 6593 |
| e-ISSN | 11782013 |
| DOI | 10.2147/ijn.s255486 |
| Journal | International Journal of Nanomedicine |
| Volume Number | ume 15 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-09-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: International Journal of Nanomedicine Micro/arc Oxidation Magnesium-incorporated Nanoporous Coating Osteoinductivity |
| Content Type | Text |
