NDLI: Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source

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Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source

Content Provider	Scilit
Author	Xu, Xin Xie, Meng Zhao, Qian Xian, Mo Liu, Huizhou
Copyright Year	2017
Description	Journal: Bioengineered We sought to produce mevalonate, an important organic acid, by recombinant Escherichia coli, using acetic acid, a less costly alternative feedstock, as a carbon source. In this study, the mevalonate biosynthesis pathway originating with acetate was constructed in recombinant E. coli, resulting in the production of 1.06 g $L^{−1}$ mevalonate with a productivity of 0.03 g $L^{−1}$ $h^{−1}$ in a 5-L bioreactor. The mevalonate concentration and productivity were significantly enhanced with increased cell density during 2-stage aerobic fermentation, reaching 7.85 g $L^{−1}$ and 0.13 g $L^{−1}$ $h^{−1}$, respectively. Fed-batch fermentation was further optimized under anaerobic and microaerobic conditions, and mevalonate concentrations reached 3.05 g $L^{−1}$ and 4.97 g $L^{−1}$, respectively, indicating that the oxygen supply exerts a large impact on mevalonate production from acetate. This study describes a method with high potential to produce mevalonate with the engineered E. coli strain XU143 using the less costly alternative feedstock acetate as a carbon source.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972924/pdf
Ending Page	123
Page Count	8
Starting Page	116
ISSN	21655979
e-ISSN	21655987
DOI	10.1080/21655979.2017.1323592
Journal	Bioengineered
Issue Number	1
Volume Number	9
Language	English
Publisher	Informa UK Limited
Publisher Date	2017-06-02
Access Restriction	Open
Subject Keyword	Journal: Bioengineered Biotechnology and Microbiology Acetate Assimilation Two-stage Fed-batch Escherichia Coli
Content Type	Text
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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