NDLI: Flow-injection sandwich ELISA for bioprocess monitoring

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Flow-injection sandwich ELISA for bioprocess monitoring

Content Provider	Scilit
Author	Rhee, Jong Il Hagedorn, Jörg Scheper, Thomas Schügerl, Karl
Copyright Year	1999
Description	Journal: Journal of Analytical Methods in Chemistry A fully automated flow-injection immunoassay based on sandwich enzyme-linked immunosorbent assay ( ELISA) is described for the model system: protein G-sepharose, rabbit IgG and horseradish peroxidase ( HRP)-labelled protein A. After injecting rabbit IgG and HRP-labelled protein A into a cartridge containing protein G-sepharose sequentially, a mixture of hydrogen peroxide and the redox indicator, 2.2-azino-bis( 3-ethylbenzthiazoline-6-sulphonic acid) ( ABT S) is passed through the cartridge. The HRPlabelled protein A bound in the cartridge is directly proportional to the concentration of rabbit IgG. The colour variation of ABTS caused during the reaction between HRP and H2O2 in the cartridge is detected photometrically. The whole assay procedure is controlled and evaluated by a computer. Rabbit IgG and HRPlabelled protein A are also detected by a fluorometer, which is introduced into the flow system. In the flow-injection sandwich ELISA, the slope of the calibration curve is 0.4491 in the range of 0 and 300mug ml-1 rabbit IgG, while it is 0.1274 in the heterogeneous immunoassay. So the flow-injection sandwich ELISA system is found to be more sensitive than a heterogeneous immunoassay for the monitoring of the model protein.
Related Links	http://europepmc.org/articles/pmc2548245?pdf=render
Ending Page	125
Page Count	5
Starting Page	121
ISSN	20908865
e-ISSN	20908873
DOI	10.1080/146392499300613
Journal	Journal of Analytical Methods in Chemistry
Issue Number	4
Volume Number	21
Language	English
Publisher	Informa UK Limited
Publisher Date	1999-07-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Analytical Methods in Chemistry Analytical Chemistry Sandwich Elisa Horseradish Peroxidase Injection Sandwich
Content Type	Text
Subject	Instrumentation Analytical Chemistry Chemical Engineering Computer Science Applications

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