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Transcriptome Analysis Reveals the Resistance Mechanism of Pseudomonas aeruginosa to Tachyplesin I
| Content Provider | Scilit |
|---|---|
| Author | Hong, Jun Jiang, Honghao Hu, Jianye Wang, Lianzhe Liu, Ruifang |
| Copyright Year | 2020 |
| Description | Journal: Infection and Drug Resistance |
| Abstract | Background: Tachyplesin I is a cationic antimicrobial peptide with a typical cyclic antiparallel β-sheet structure. We previously demonstrated that long-term continuous exposure to increased concentration of tachyplesin I can induce resistant Gram-negative bacteria. However, no significant information is available about the resistance mechanism of Pseudomonas aeruginosa (P. aeruginosa) to tachyplesin I. Materials and Methods: In this study, the global gene expression profiling of P. aeruginosa strain PA-99 and P. aeruginosa CGMCC1.2620 (PA1.2620) was conducted using transcriptome sequencing. For this purpose, outer membrane permeability and outer membrane proteins (OMPs) were further analyzed. Results: Transcriptome sequencing detected 672 upregulated and 787 downregulated genes, covering Clusters of Orthologous Groups (COGs) of P. aeruginosa strain PA-99 compared with PA1.2620. Totally, 749 differentially expressed genes (DEGs) were assigned to 98 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and among them, a two-component regulatory system, a beta-lactam resistance system, etc. were involved in some known genes resistant to drugs. Additionally, we further attempted to indicate whether the resistance mechanism of P. aeruginosa to tachyplesin I was associated with the changes of outer membrane permeability and OMPs. Conclusion: Our results indicated that P. aeruginosa resistant to tachyplesin I was mainly related to reduced entry of tachyplesin I into the bacterial cell due to overexpression of efflux pump, in addition to a decrease of outer membrane permeability. Our findings were also validated by pathway enrichment analysis and quantitative reverse transcription polymerase chain reaction (RT-qPCR). This study may provide a promising guidance for understanding the resistance mechanism of P. aeruginosa to tachyplesin I. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6970625/pdf https://www.dovepress.com/getfile.php?fileID=55462 |
| Ending Page | 169 |
| Page Count | 15 |
| Starting Page | 155 |
| ISSN | 11786973 |
| e-ISSN | 11786973 |
| DOI | 10.2147/idr.s226687 |
| Journal | Infection and Drug Resistance |
| Volume Number | ume 13 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Infection and Drug Resistance Integrative and Complementary Medicine Antibiotic Resistance Tachyplesin I P. Aeruginosa Differentially Expressed Genes Outer Membrane Permeability Outer Membrane Proteins |
| Content Type | Text |
| Subject | Infectious Diseases Pharmacology Pharmacology (medical) |
