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Intermedin protects HUVECs from ischemia reperfusion injury via Wnt/β-catenin signaling pathway
| Content Provider | Scilit |
|---|---|
| Author | Wang, Yanhong Wu, Zhijing Tian, Jihua Mi, Yang Ren, Xiaojun Kang, Jing Zhang, Wan Zhou, Xiaoshuang Wang, Guiqin Li, Rongshan |
| Copyright Year | 2019 |
| Description | Journal: Renal Failure Intermedin (IMD) is a member of the calcitonin gene-related peptide (CGRP) superfamily and a pro-angiogenic factor. In the present study, we identified activation of the Wnt/β-catenin signaling pathway by IMD. Adding $CoCl_{2}$ HUVECs was used to establish an in vitro model. The migration of HUVECs was measured by wound healing assays and transwell migration assays. Capillary formation was measured using tube formation assays. Immunocytochemistry (ICC) analysis was used to evaluate VEGF and RAMP2 expression in HUVECs. The relevant signaling molecules were detected with western blot. Our study shows that IMD could promote H/R impaired HUVECs migration and tube formation in vitro. On the other hand, inhibition of Wnt/β-catenin signaling led to the suppression of this promotion of migration and tube formation. This result suggests that Wnt/β-catenin signaling is correlated to IMD induced angiogenesis. Analysis of results from ICC assays indicated that IMD works through increasing levels of VEGF and RAMP2. Meanwhile, the Wnt/β-catenin signaling specific inhibitor IWR-1-endo was shown to down-regulate VEGF and RAMP2 expression. Western blot results further confirmed the signaling mechanism by which IMD promotes angiogenesis. Thus, Wnt/β-catenin signaling plays an important role in IMD induced neovascularization. The data further suggest that the PI3K axis contributes positively downstream. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/0886022X.2019.1587468?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450471/pdf |
| Ending Page | 166 |
| Page Count | 8 |
| Starting Page | 159 |
| ISSN | 0886022X |
| e-ISSN | 15256049 |
| DOI | 10.1080/0886022x.2019.1587468 |
| Journal | Renal Failure |
| Issue Number | 1 |
| Volume Number | 41 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2019-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Renal Failure Biochemistry and Molecular Biology Angiogenesis Wnt/β-catenin Signaling Hypoxia/reoxygenation |
| Content Type | Text |
| Subject | Critical Care and Intensive Care Medicine Nephrology |
